用于生物人工组织生成的3D明胶支架的激光制造
Laser Fabrication of 3D Gelatin Scaffolds for the Generation of Bioartificial Tissues.
作者信息
Ovsianikov Aleksandr, Deiwick Andrea, Van Vlierberghe Sandra, Pflaum Michael, Wilhelmi Mathias, Dubruel Peter, Chichkov Boris
机构信息
Department of Nanotechnology, Laser Zentrum Hannover e.V., Hollerithallee 8, D-30419 Hannover, Germany.
Polymer Chemistry & Biomaterials Research Group, University of Ghent, Krijgslaan 281, Building S4-Bis, 9000 Ghent, Belgium.
出版信息
Materials (Basel). 2011 Jan 19;4(1):288-299. doi: 10.3390/ma4010288.
Abstract
In the present work, the two-photon polymerization (2PP) technique was applied to develop precisely defined biodegradable 3D tissue engineering scaffolds. The scaffolds were fabricated via photopolymerization of gelatin modified with methacrylamide moieties. The results indicate that the gelatin derivative (GelMod) preserves its enzymatic degradation capability after photopolymerization. In addition, the developed scaffolds using 2PP support primary adipose-derived stem cell (ASC) adhesion, proliferation and differentiation into the anticipated lineage.
摘要
在本研究中，采用双光子聚合（2PP）技术制备了具有精确结构的可生物降解三维组织工程支架。通过甲基丙烯酰胺修饰的明胶光聚合制备了支架。结果表明，明胶衍生物（GelMod）在光聚合后仍保留其酶降解能力。此外，使用2PP技术制备的支架支持原代脂肪来源干细胞（ASC）的黏附、增殖并分化为预期的细胞谱系。
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