Crispie Fiona, Twomey Denis, Flynn James, Hill Colin, Ross Paul, Meaney William
Teagasc, Dairy Products Research Centre, Moorepark, Fermoy, Co. Cork.
J Dairy Res. 2005 May;72(2):159-67. doi: 10.1017/s0022029905000816.
A preparation of the bacteriocin lacticin 3147 (prepared from a demineralized whey protein fermentation liquor) was combined as a powder with a bismuth-based intramammary teat seal and evaluated for its potential as an antimicrobial in non-lactating cows. The lacticin/teat seal formulation enabled significant bacteriocin release from the seal without the requirement for a surfactant. Studies in vivo in lactating cows demonstrated that this formulation was effective in reducing bacterial recoveries (approximately 20-fold) from teats deliberately inoculated with Staphylococcus aureus after infusion. Moreover, this formulation also significantly reduced the numbers of Staph. aureus recovered from teats that were exposed to the challenge bacterium before the infusion of the teat seal preparation. The powdered preparation of lacticin 3147 did, however, cause some teat irritation as evidenced by associated rises in somatic cell count (SCC). However, this effect was short-lived and when the mean SCC readings pre-infusion and the final two readings post-infusion were compared, there was no significant difference in the immunological acceptance between treatments.
将一种细菌素乳酸链球菌素3147制剂（由脱矿质乳清蛋白发酵液制备）作为粉末与铋基乳房内乳头封闭剂混合，并评估其在非泌乳奶牛中作为抗菌剂的潜力。乳酸链球菌素/乳头封闭剂配方能够在无需表面活性剂的情况下从封闭剂中大量释放细菌素。对泌乳奶牛进行的体内研究表明，该配方在减少故意接种金黄色葡萄球菌的乳头的细菌回收率（约20倍）方面是有效的。此外，该配方还显著减少了在注入乳头封闭剂制剂之前暴露于挑战细菌的乳头中回收的金黄色葡萄球菌数量。然而，乳酸链球菌素3147的粉末制剂确实引起了一些乳头刺激，体细胞计数（SCC）的相关升高证明了这一点。然而，这种影响是短暂的，当比较输注前的平均SCC读数和输注后的最后两个读数时，各处理之间的免疫接受度没有显著差异。
