Sharma S N, Shukla R P, Mittal P K, Adak T, Kumar A
Malaria Research Centre, Field Station, Inderjeet Garden, Bhatia Parao, Haldwani, District Nainital, Uttaranchal- 263141.
J Commun Dis. 2003 Dec;35(4):290-9.
Efficacy of a new Bti formulation was tested in the laboratory and small scale field trials against mosquito larvae were carried out from March to June, 2001 in different breeding habitats of Kumaun foothill region of Nainital and Udham Singh Nagar districts, Uttaranchal state, India. Laboratory tests revealed increased efficacy against Culex quinquefasciatus (LC50 = 0.035 mg/L) followed by Aedes aegypti (LC50 = 0.0628 mg/L), An. culicifacies sp A (LC50 = 0.184mg/L) and An. stephensi (LC50 - 0.2216mg/L). Results of field trials revealed 100% mortality of mosquito larvae after treatment with Bti @ 0.5 gm/m2 surface area. The impact was similar on different mosquito species breeding in different habitats. However, repeated treatments were required due to reappearance of larvae in the breeding habitats within a week. No side effects of Bti was observed during field trial on non- target organisms (NTOs). Environmental disturbances and man made problems affected the spray impact of Bti.
2001年3月至6月，在印度北阿坎德邦纳依纳特尔和乌德汉姆·辛格·纳加尔地区库马盎山麓地区的不同蚊虫滋生地，对一种新型苏云金芽孢杆菌以色列亚种（Bti）制剂进行了实验室测试，并开展了针对蚊虫幼虫的小规模田间试验。实验室测试表明，该制剂对致倦库蚊（LC50 = 0.035毫克/升）的杀灭效果最佳，其次是埃及伊蚊（LC50 = 0.0628毫克/升）、库氏按蚊A种（LC50 = 0.184毫克/升）和斯氏按蚊（LC50 = 0.2216毫克/升）。田间试验结果显示，以每平方米表面积0.5克的剂量施用Bti后，蚊虫幼虫的死亡率达到100%。对于在不同栖息地滋生的不同蚊虫种类，效果相似。然而，由于一周内滋生地内幼虫再次出现，需要进行重复处理。在田间试验期间，未观察到Bti对非靶标生物有任何副作用。环境干扰和人为问题影响了Bti的喷洒效果。
