Zhang Hai-Ying, Nanji Amin A, Luk John M, Huang Xiao-Ru, Lo Chung-Mau, Chen Yong Xiong, Yuen Siu-Tsan, Lan Hui Y, Lau George K K
Department of Medicine, The University of Hong Kong, Hong Kong, SAR, China.
Liver Int. 2005 Jun;25(3):571-9. doi: 10.1111/j.1478-3231.2005.01047.x.
Macrophage migration inhibitory factor (MIF) has emerged to be a pivotal cytokine in immune-mediated diseases.
To investigate the role of MIF in chronic hepatitis B infection, we studied two groups of hepatitis B surface antigen positive patients: group 1 (immune tolerant, n = 16) and group 2 (immune clearance, n = 16). Serum level of MIF was measured by enzyme-linked immunosorbent assay and intrahepatic expression of MIF, macrophage and T-cell localisation were detected by double immunohistochemistry.
An increased serum MIF correlated significantly with increased serum alanine aminotransferase activity (r = 0.73, P < 0.001) and the severity of necroinflammatory injury (r = 0.642, P < 0.001). In group 2, there was marked MIF mRNA expression in all KP-1+ macrophages and CD45RO+ activated T cells and, to a lesser extent, in hepatocytes within inflammatory areas. In contrast to its mRNA expression, the cytoplasmic MIF protein level in hepatocytes, infiltrating macrophages and T cells within the inflammatory area was reduced, which probably contributed to the increased serum MIF level.
Our data suggested that MIF played a role in sustaining cell-mediated hepatic injury during the immune-clearance phase of chronic hepatitis B infection.
巨噬细胞移动抑制因子（MIF）已成为免疫介导疾病中的一种关键细胞因子。
为研究MIF在慢性乙型肝炎感染中的作用，我们研究了两组乙型肝炎表面抗原阳性患者：第1组（免疫耐受，n = 16）和第2组（免疫清除，n = 16）。通过酶联免疫吸附测定法测量血清MIF水平，并通过双重免疫组织化学检测肝内MIF表达、巨噬细胞和T细胞定位。
血清MIF升高与血清丙氨酸氨基转移酶活性增加显著相关（r = 0.73，P < 0.001）以及坏死性炎症损伤的严重程度相关（r = 0.642，P < 0.001）。在第2组中，所有KP - 1 +巨噬细胞和CD45RO +活化T细胞中均有明显的MIF mRNA表达，在炎症区域内的肝细胞中表达程度较低。与其mRNA表达相反，炎症区域内肝细胞、浸润的巨噬细胞和T细胞中的细胞质MIF蛋白水平降低，这可能导致血清MIF水平升高。
我们的数据表明，MIF在慢性乙型肝炎感染的免疫清除阶段维持细胞介导的肝损伤中发挥作用。
