Hwang Jee Youn, Markkandan Kesavan, Han Kyudong, Kwon Mun Gyeong, Seo Jung Soo, Yoo Seung-Il, Hwang Seong Don, Ji Bo Young, Son Maeng-Hyun, Park Jun-Hyung
Aquatic Disease Control Division, National Institute of Fisheries Science (NIFS), 216 Gijanghaean-Ro, Gijang-up, Gijang-Gun, Busan, 46083, Republic of Korea.
TheragenETEX Bio Institute, TheragenETEX Inc., Suwon, 16229, Republic of Korea.
Genes Genomics. 2018 Mar;40(3):315-320. doi: 10.1007/s13258-017-0638-0. Epub 2017 Dec 1.
Olive flounder (Paralichthys olivaceus) is one of the most economically important aquaculture fish. However, its production is often affected by various diseases, especially viral hemorrhagic septicemia virus (VHSV) that cause serious economic losses. In this study, we sequenced the whole transcriptome of the P. olivaceus using Illumina RNA-sEq. De novo assembly of control and virus-infected cDNA libraries of head kidney at 13 and 20 °C was accomplished with 2,007,532,438 raw reads, resulting in 244,578 unigenes with an average length of 533 bp and found 65,535 candidate coding unigenes with homology to other species by BLAST analysis. DEG analysis among control and virus-infected head kidney samples of 13 and 20 °C revealed that 1290 up-regulated and 162 down-regulated genes (p ≤ 0.01), linked to metabolism, virulence factors, adhesion and immune-response. We constructed an expressed gene catalog for the P. olivaceus to serve as a resource for marine environmental genomic and immuno-genetic/genomic studies focused on uncovering the molecular mechanisms underlying the responses of P. olivaceus to VHSV under different temperature.
牙鲆（Paralichthys olivaceus）是经济价值最重要的水产养殖鱼类之一。然而，其产量常受各种疾病影响，尤其是病毒性出血性败血症病毒（VHSV），会造成严重经济损失。在本研究中，我们使用Illumina RNA-sEq对牙鲆的全转录组进行了测序。利用2,007,532,438条原始读数对13℃和20℃下对照及病毒感染的头肾cDNA文库进行了从头组装，得到244,578个单基因，平均长度为533 bp，并通过BLAST分析发现了65,535个与其他物种具有同源性的候选编码单基因。对13℃和20℃下对照及病毒感染的头肾样本进行差异表达基因（DEG）分析，发现1290个上调基因和162个下调基因（p≤0.01），这些基因与代谢、毒力因子、黏附及免疫反应相关。我们构建了牙鲆的表达基因目录，作为海洋环境基因组学以及专注于揭示牙鲆在不同温度下对VHSV反应潜在分子机制的免疫遗传/基因组学研究的资源。
