Balkrishna Acharya, Sakat Sachin Shridhar, Joshi Kheemraj, Joshi Kamal, Sharma Vinay, Ranjan Ravikant, Bhattacharya Kunal, Varshney Anurag
Drug Discovery and Development Division, Patanjali Research Institute, Haridwar, India.
Department of Allied Sciences, University of Patanjali, Patanjali YogPeeth, Haridwar, India.
Front Pharmacol. 2019 Oct 11;10:1186. doi: 10.3389/fphar.2019.01186. eCollection 2019.
Psoriasis is a chronic inflammatory skin disease characterized by circumscribed, red, thickened plaques with overlying silvery white scales. It is associated with the release of pro-inflammatory mediators that lead to the development of edema and distress. Here we show the anti-inflammatory and anti-psoriatic efficacies of a neutraceutical sea buckthorn oil (SBKT) derived from the fruit pulp of . Chemical analysis of the SBKT showed the presence of 16 major saturated, mono-, and polyunsaturated fatty acids components, imparting significant nutritional values. Efficacy of the SBKT in modulating psoriasis and associated inflammation was first tested using human monocytic (THP-1) cells. SBKT induced cytotoxicity at a dose of ≥25 µl/ml. Treatment of the lipopolysaccharide-stimulated THP-1 cells with SBKT subdued the enhanced release of intracellular reactive nitrogen species and expression of NF-κB protein, in a concentration-dependent manner. This was accompanied by a reduction in the release of downstream pro-inflammatory cytokines: Interleukin-1ß and interleukin-6. Tumor necrosis factor-α released in the stimulated THP-1 cells were also inhibited by SBKT dose of 5 µl/ml. oral and topical treatment with SBKT in the Carrageenan-stimulated paw edema model, showed a significant decrease in paw volume and edema. In the 12-O tetradecanoyl phorbol 13-acetate (TPA) stimulated CD-1 mice psoriasis-like model, concurrent oral and tropical SBKT treatments substantially reduced ear edema and ear biopsy weights. Histopathologically, significant reduction in ear epidermal thickness and skin lesion scores was observed in the SBKT-treated animals. In conclusion, SBKT showed anti-inflammatory and anti-psoriasis-like efficacies in healing chemical-induced inflammation and psoriasis. The possible mode of action of SBKT was found through inhibition of reactive nitrogen species, and downregulation of NF-κB protein and pro-inflammatory cytokines. Thus, the present data suggest that Sea buckthorn oil can be used as an anti-inflammatory and anti-psoriatic nutraceutical.
银屑病是一种慢性炎症性皮肤病，其特征为边界清晰、发红、增厚的斑块，上面覆盖着银白色鳞屑。它与促炎介质的释放有关，这些介质会导致水肿和不适。在此，我们展示了一种从沙棘果肉中提取的营养保健品沙棘油（SBKT）的抗炎和抗银屑病功效。对SBKT的化学分析表明，其含有16种主要的饱和、单不饱和和多不饱和脂肪酸成分，具有显著的营养价值。首先使用人单核细胞（THP-1）细胞测试了SBKT在调节银屑病及相关炎症方面的功效。SBKT在剂量≥25微升/毫升时会诱导细胞毒性。用SBKT处理脂多糖刺激的THP-1细胞，以浓度依赖的方式抑制了细胞内活性氮物质的释放增加和NF-κB蛋白的表达。这伴随着下游促炎细胞因子白细胞介素-1β和白细胞介素-6释放的减少。在刺激的THP-1细胞中释放的肿瘤坏死因子-α也被5微升/毫升剂量的SBKT所抑制。在角叉菜胶刺激的爪水肿模型中，SBKT的口服和局部治疗显示爪体积和水肿显著减少。在12-O-十四烷酰佛波醇-13-乙酸酯（TPA）刺激的CD-1小鼠银屑病样模型中，同时进行口服和局部SBKT治疗可显著减轻耳部水肿和耳部活检重量。组织病理学上，在接受SBKT治疗的动物中观察到耳部表皮厚度和皮肤病变评分显著降低。总之，SBKT在治愈化学诱导的炎症和银屑病方面显示出抗炎和抗银屑病样功效。通过抑制活性氮物质以及下调NF-κB蛋白和促炎细胞因子，发现了SBKT可能的作用方式。因此，目前的数据表明沙棘油可作为一种抗炎和抗银屑病的营养保健品。
