Gupta Asheesh, Kumar Ratan, Pal Karan, Singh Virendra, Banerjee Pratul K, Sawhney Ramesh C
Defence Institute of Physiology and Allied Sciences, Lucknow Road, Timarpur, Delhi 110054, India.
Mol Cell Biochem. 2006 Oct;290(1-2):193-8. doi: 10.1007/s11010-006-9187-6. Epub 2006 Apr 22.
The present investigation was undertaken to determine the efficacy of topical administration of flavone of sea buckthorn (Hippophae rhamnoides L.) on cutaneous wound healing in rats. Four full-thickness excision wounds were created on the back of rat and 1.0% w/v flavone prepared in propylene glycol was applied topically. Control animals received the vehicle alone in an identical manner. The healing of the wound was assessed by the rate of wound contraction, period of epithelialization, hydroxyproline, hexosamine, antioxidants estimation and histopathology of the granulation tissue. The sea buckthorn flavone promoted the wound healing activity as indicated by improved rate of wound contraction, decreased time taken for epithelialization (16.3 days versus 24.8 days in controls) and significant increase in hydroxyproline (26.0%) and hexosamine (30.0%) content. These findings were also confirmed by histopathological examinations. In addition, it was observed that sea buckthorn flavone possesses potent antioxidant properties as evidenced by significant increase in reduced glutathione (55.0%), vitamin C (70.0%) and catalase (20.0%) activities in wound granulation tissue. The flavone treatment also resulted in significant decrease in lipid peroxide levels (39.0%). The results suggest that the sea buckthorn flavone promotes wound healing activity.
本研究旨在确定局部应用沙棘黄酮（沙棘）对大鼠皮肤伤口愈合的疗效。在大鼠背部制造四个全层切除伤口，并局部应用在丙二醇中制备的1.0%（w/v）黄酮。对照动物以相同方式仅接受赋形剂。通过伤口收缩率、上皮化时间、羟脯氨酸、己糖胺、抗氧化剂估计以及肉芽组织的组织病理学来评估伤口的愈合情况。沙棘黄酮促进了伤口愈合活性，表现为伤口收缩率提高、上皮化所需时间减少（16.3天对对照组的24.8天）以及羟脯氨酸（26.0%）和己糖胺（30.0%）含量显著增加。这些发现也通过组织病理学检查得到证实。此外，观察到沙棘黄酮具有强大的抗氧化特性，伤口肉芽组织中还原型谷胱甘肽（55.0%）、维生素C（70.0%）和过氧化氢酶（20.0%）活性显著增加证明了这一点。黄酮处理还导致脂质过氧化物水平显著降低（39.0%）。结果表明沙棘黄酮促进伤口愈合活性。
