Tymon Anna M, Pell Judith K
Plant and Invertebrate Ecology Division, Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK.
Mycol Res. 2005 Mar;109(Pt 3):285-93. doi: 10.1017/s0953756204001807.
The entomopathogenic fungus Pandora neoaphidis is an important natural enemy of aphids. ISSR, ERIC (Enterobacterial Repetitive Intergenic Consensus) and RAPD PCR-based DNA fingerprint analyses were undertaken to study intra-specific variation amongst 30 isolates of P. neoaphidis worldwide, together with six closely related species of Entomophthorales. All methods yielded scorable binary characters, and distance matrices were constructed from both individual and combined data sets. Neighbour-joining was used to construct consensus phylogenetic trees which showed that although P. neoaphidis isolates were highly polymorphic they separated into a monophyletic group compared with the other Entomophthorales tested. Three distinct subclades were found, with UK isolates occupying two of these. No specific correlation with aphid host species was established for any of the isolates apart from those in one cluster which contained isolates obtained from nettle aphid, Microlophium carnosum. ERIC, ISSR and RAPD analysis allowed the rapid genetic characterisation and differentiation of isolates with the generation of potential isolate- and cluster specific-diagnostic DNA markers.
昆虫病原真菌新蚜虫疠霉是蚜虫的一种重要天敌。本研究采用基于ISSR、ERIC（肠杆菌重复基因间共有序列）和RAPD的PCR DNA指纹分析方法，对全球范围内的30株新蚜虫疠霉分离株以及6种相近的虫霉目真菌进行种内变异研究。所有方法均产生了可评分的二元性状，并根据单个和组合数据集构建了距离矩阵。采用邻接法构建了共识系统发育树，结果表明，尽管新蚜虫疠霉分离株具有高度多态性，但与其他受试虫霉目真菌相比，它们聚为一个单系类群。发现了三个不同的亚分支，其中英国的分离株占据了其中两个。除了一个包含从荨麻蚜（Microlophium carnosum）获得的分离株的簇中的分离株外，未发现任何分离株与蚜虫宿主物种存在特定相关性。ERIC、ISSR和RAPD分析能够通过生成潜在的分离株和簇特异性诊断DNA标记，快速对分离株进行遗传特征鉴定和区分。
