Aquino de Muro Marilena, Elliottt Sarah, Moore David, Parker Bruce L, Skinner Margaret, Reid William, El Bouhssini Mustapha
CABI Bioscience UK Centre, Bakeham Lane, Egham TW20 9TY, UK.
Mycol Res. 2005 Mar;109(Pt 3):294-306. doi: 10.1017/s0953756204001832.
110 isolates of Beauveria (104 B. bassiana, 5 Beauveria spp., 1 B. brongniartii) were obtained from Sunn Pests (Eurygaster and Aelia species), litter, and other insect samples at overwintering sites in seven countries in the Middle East and West Asia. DNA was extracted from these isolates, and four techniques were used to characterize and to investigate genetic diversity at the molecular level: ITS-RFLP, ITS sequencing, ISSR-PCR, and AFLP. The ITS-RFLP and ITS sequences did not detect significant genetic variation among the isolates. However, both ISSR-PCR and AFLP analyses gave indications of intraspecific groupings correlated with geographical origin and relative genetic diversity among some isolates, but no obvious association with Sunn Pest hosts. There was no obvious genotypic grouping of B. bassiana isolates from E. integriceps, perhaps suggesting the overwintering populations were infected by generalist native isolates rather than by host-specific ones that might be more suitable for biocontrol purposes.
从位于中东和西亚七个国家越冬地点的麦蝽（Eurygaster和Aelia属物种）、枯枝落叶以及其他昆虫样本中获得了110株白僵菌分离株（104株球孢白僵菌、5株白僵菌属其他种、1株布氏白僵菌）。从这些分离株中提取DNA，并使用四种技术在分子水平上进行特征分析和遗传多样性研究：ITS-RFLP、ITS测序、ISSR-PCR和AFLP。ITS-RFLP和ITS序列未检测到分离株之间存在显著的遗传变异。然而，ISSR-PCR和AFLP分析均显示，种内分组与地理起源以及一些分离株之间的相对遗传多样性相关，但与麦蝽宿主没有明显关联。来自Integriceps麦蝽的球孢白僵菌分离株没有明显的基因型分组，这可能表明越冬种群是被本地的泛化型分离株感染，而非可能更适合生物防治目的的宿主特异性分离株。
