Kasamo M, Brandt M, Ishikawa M, Shimizu T, Harper M J
Department of Obstetrics and Gynecology, University of Texas Health Science Center, San Antonio 78284-7836.
Biol Reprod. 1992 May;46(5):829-45. doi: 10.1095/biolreprod46.5.829.
Prostaglandin (PG) release from and platelet-activating factor (PAF) accumulation by enzymatically isolated endometrial epithelial and stromal cells from Day 6 pregnant and Day 6 pseudopregnant rabbits were studied in vitro, using RIA for PG measurement and a platelet aggregation assay for PAF measurement. On the first day of culture in serum-free media, PGF release into the medium was significantly higher from epithelial cells from Day 6 of pregnancy than from stromal cells from Day 6 of pregnancy or pseudopregnancy. PGE release did not differ significantly among these cell types. The addition of indomethacin (10(-5) M) to similar cultures inhibited release of both PGs from both cell types, but to a much greater extent from stromal than from epithelial cells. Significant stimulation of PG release by A23187 was achieved under all conditions on the fifth day of culture; PGE release was significantly greater than PGF release from stromal cells from Day 6 of pregnancy and pseudopregnancy, and release of both PGs from stromal cells was significantly greater from Day 6 of pregnancy than from Day 6 of pseudopregnancy. PG release from similar cells, cultured in medium containing 10% calf serum, was highest on the first or second day of culture and then, especially for PGF, declined with continued culture. PGE release was significantly higher than PGF release from stromal cells on the third and fourth days of culture. The ratios of PGF/PGE release from epithelial cells were significantly higher than those from stromal cells over the 5-day culture period for both reproductive stages. These ratios indicate the differential release of PGE and PGF from rabbit endometrial cell subpopulations and indicate a preferential release of PGE from stromal and of PGF from epithelial cells. Under basal conditions, PAF was not detected in epithelial or stromal cells cultured for 2 or 4 days, or in the associated culture media. If PAF had been released into the medium, it would have rapidly metabolized. Short exposure to calcium ionophore A23187 (10(-5) M) was able to stimulate PAF accumulation in epithelial and stroma cells in serum-free media, probably via the remodeling pathway. PAF was not detected in the medium. Intracellular PAF accumulation after exposure to A23187 (10(-5) M) for 5 min was significantly greater on the second day of culture than on the fourth day in epithelial and stromal cells from Day 6 of pregnancy.(ABSTRACT TRUNCATED AT 400 WORDS)
采用放射免疫分析法测定前列腺素（PG）以及血小板聚集试验测定血小板活化因子（PAF），对酶解分离的妊娠第6天和假孕第6天兔子宫内膜上皮细胞和基质细胞释放PG及PAF的情况进行了体外研究。在无血清培养基中培养的第一天，妊娠第6天的上皮细胞释放到培养基中的PGF显著高于妊娠第6天或假孕第6天的基质细胞。这些细胞类型之间PGE的释放没有显著差异。在类似培养物中加入吲哚美辛（10⁻⁵M）可抑制两种细胞类型中PG的释放，但对基质细胞的抑制作用比对上皮细胞大得多。在培养的第五天，在所有条件下A23187均能显著刺激PG的释放；妊娠第6天和假孕第6天的基质细胞释放的PGE显著大于PGF，且妊娠第6天基质细胞释放的两种PG均显著大于假孕第6天。在含10%小牛血清的培养基中培养的类似细胞，在培养的第一天或第二天PG释放最高，然后，尤其是PGF，随着继续培养而下降。在培养的第三天和第四天，基质细胞释放的PGE显著高于PGF。在两个生殖阶段的5天培养期内，上皮细胞释放的PGF/PGE比值显著高于基质细胞。这些比值表明兔子宫内膜细胞亚群中PGE和PGF的释放存在差异，表明基质细胞优先释放PGE，上皮细胞优先释放PGF。在基础条件下，培养2天或4天的上皮细胞或基质细胞及其相关培养基中均未检测到PAF。如果PAF已释放到培养基中，它会迅速代谢。短时间暴露于钙离子载体A23187（10⁻⁵M）能够刺激无血清培养基中上皮细胞和基质细胞内PAF的积累，可能是通过重塑途径。培养基中未检测到PAF。暴露于A23187（10⁻⁵M）5分钟后，妊娠第6天上皮细胞和基质细胞在培养第二天的细胞内PAF积累显著大于第四天。（摘要截断于400字）
