Atsuta Ikiru, Yamaza Takayoshi, Yoshinari Masao, Goto Tetsuya, Kido Mizuho A, Kagiya Tadayoshi, Mino Satoya, Shimono Masaki, Tanaka Teruo
Department of Oral Anatomy and Cell Biology, Kyushu University Graduate School of Dental Science, Fukuoka, Japan.
Biomaterials. 2005 Nov;26(32):6280-7. doi: 10.1016/j.biomaterials.2005.03.046.
Laminin-5 (Ln-5) is an important molecule associated with epithelial cell adhesion and migration. In the gingiva around the tooth, Ln-5 localizes within basement membranes between the junctional epithelium (JE) and the tooth or connective tissue. Recently, we reported that in the oral mucosa around a dental implant, Ln-5 is expressed within the basement membranes at the implant-peri-implant epithelium (PIE) interface, and at the PIE-connective tissue interface. However, the ultrastructural localization of Ln-5 within or along the PIE has not yet been reported. Therefore, peri-implant oral mucosa was treated with anti-Ln-5 (gamma2 chain) antibody and examined using immuno-electron microscopy. Ln-5 was localized in the cells of the innermost-third layer and basal layer of the PIE. A 100-nm-wide Ln-5-positive internal basal lamina (basement membrane) and hemidesmosomes as adhesion structures were formed at the apical portion of the implant-PIE interface. However, at the upper-middle portion of the interface, these adhesion structures were not observed. Furthermore, at the PIE-connective tissue interface, the Ln-5-positive external basal lamina (basement membrane) and hemidesmosomes were partially deficient. Judging from these findings, we concluded that Ln-5 contributes to the attachment of the PIE to the titanium surface, and that PIE attached to titanium at the apical portion of the dental implant-PIE interface.
层粘连蛋白-5（Ln-5）是一种与上皮细胞黏附和迁移相关的重要分子。在牙齿周围的牙龈中，Ln-5定位于结合上皮（JE）与牙齿或结缔组织之间的基底膜内。最近，我们报道在牙种植体周围的口腔黏膜中，Ln-5在种植体-种植体周围上皮（PIE）界面以及PIE-结缔组织界面的基底膜内表达。然而，Ln-5在PIE内或沿PIE的超微结构定位尚未见报道。因此，用抗Ln-5（γ2链）抗体处理种植体周围口腔黏膜，并使用免疫电子显微镜进行检查。Ln-5定位于PIE最内层的三分之一层和基底层的细胞中。在种植体-PIE界面的顶端部分形成了一个宽100nm的Ln-5阳性内基底膜（基底膜）和作为黏附结构的半桥粒。然而，在界面的中上部，未观察到这些黏附结构。此外，在PIE-结缔组织界面，Ln-5阳性外基底膜（基底膜）和半桥粒部分缺失。根据这些发现，我们得出结论，Ln-5有助于PIE与钛表面的附着，并且PIE在牙种植体-PIE界面的顶端部分附着于钛。
