Postiglione L, Spanò A, Varricchio P, Larizza G, Oriente A, Gattozzi D, Oriente P
Department of Internal Medicine, 2nd Medical School, University of Naples, Italy.
Int J Clin Lab Res. 1992;21(4):318-20. doi: 10.1007/BF02591670.
Epidemiological and clinical evidence have indicated that apolipoprotein A1 and B determination can better define the lipoprotein pattern in normal subjects and in subjects with coronary heart disease. In this paper, a recent immunoturbidimetric method for routine apolipoprotein A1 and B measurement (using the Turbitimer system and commercially available antisera) has been evaluated. The precision and the accuracy of the method have been previously considered. Within-run and between-run coefficients of variation (ranging from 1.67% to 5.04%) for both assays indicate good precision of the method. Accuracy was evaluated on 2 consecutive days (n = 10 each run) using a standard serum for apolipoprotein A1 and B. The bias obtained was 3.79% for apolipoprotein A1 and 2.30% for B. Apolipoproteins A1 and B were then measured in 100 normal and hyperlipemic sera with the immunoturbidimetric assay and radial immunodiffusion (using the monoclonal antibodies). The data obtained were evaluated by linear regression analysis (Al, r = 0.893; B, r = 0.862). The good correlation between the two methods suggests that the immunoturbidimetric assay can be usefully performed for routine apolipoprotein A1 and B determination because of its lower cost, rapidity, and simplicity.
流行病学和临床证据表明，载脂蛋白A1和B的测定能够更好地确定正常人和冠心病患者的脂蛋白模式。本文对一种用于常规测定载脂蛋白A1和B的免疫比浊法（使用Turbitimer系统和市售抗血清）进行了评估。该方法的精密度和准确性已在之前进行了考量。两种检测的批内和批间变异系数（范围为1.67%至5.04%）表明该方法具有良好的精密度。使用载脂蛋白A1和B的标准血清连续两天（每次检测n = 10）评估准确性。载脂蛋白A1的偏差为3.79%，载脂蛋白B的偏差为2.30%。然后使用免疫比浊法和放射免疫扩散法（使用单克隆抗体）对100份正常和高脂血症血清中的载脂蛋白A1和B进行测定。通过线性回归分析对所得数据进行评估（A1，r = 0.893；B，r = 0.862）。两种方法之间的良好相关性表明，免疫比浊法因其成本较低、速度快且操作简单，可有效地用于常规载脂蛋白A1和B的测定。
