Nakari Tarja
Finnish Environment Institute, Hakuninmaantie 6, FIN 00430 Helsinki, Finland.
Environ Sci. 2005;12(2):87-97.
The estrogenic activity of two phytosterol preparations was evaluated in vitro and in vivo. For the in vitro evaluation, freshly separated hepatocytes of rainbow trout were used. By contrast, the in vivo evaluation was performed by injecting the phytosterols intraperitoneally into juvenile rainbow trout. Both assays confirmed the estrogenic activity of the phytosterols. The in vitro screening technique, based on the synthesis and secretion of vitellogenin from the isolated liver cells, produced a clear, significant curve in response to the presence of both phytosterol mixtures. In the in vivo tests, the phytosterol preparations caused significant increases in plasma vitellogenin concentrations of juvenile fish. These shortterm assays proved to be suitable for assessing the estrogenic activity of phytosterols.
对两种植物甾醇制剂的雌激素活性进行了体外和体内评估。体外评估使用了刚分离出的虹鳟肝细胞。相比之下，体内评估是通过将植物甾醇腹腔注射到幼年虹鳟中来进行的。两种测定均证实了植物甾醇的雌激素活性。基于从分离的肝细胞中合成和分泌卵黄蛋白原的体外筛选技术，在两种植物甾醇混合物存在的情况下产生了清晰、显著的曲线。在体内试验中，植物甾醇制剂导致幼鱼血浆中卵黄蛋白原浓度显著增加。这些短期试验被证明适用于评估植物甾醇的雌激素活性。
