Walker Glenn M, Sai Jiqing, Richmond Ann, Stremler Mark, Chung Chang Y, Wikswo John P
Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt University, Nashville, TN 37235, USA.
Lab Chip. 2005 Jun;5(6):611-8. doi: 10.1039/b417245k. Epub 2005 Apr 27.
An understanding of chemotaxis at the level of cell-molecule interactions is important because of its relevance in cancer, immunology, and microbiology, just to name a few. This study quantifies the effects of flow on cell migration during chemotaxis in a microfluidic device. The chemotaxis gradient within the device was modeled and compared to experimental results. Chemotaxis experiments were performed using the chemokine CXCL8 under different flow rates with human HL60 promyelocytic leukemia cells expressing a transfected CXCR2 chemokine receptor. Cell trajectories were separated into x and y axis components. When the microchannel flow rates were increased, cell trajectories along the x axis were found to be significantly affected (p < 0.05). Total migration distances were not affected. These results should be considered when using similar microfluidic devices for chemotaxis studies so that flow bias can be minimized. It may be possible to use this effect to estimate the total tractile force exerted by a cell during chemotaxis, which would be particularly valuable for cells whose tractile forces are below the level of detection with standard techniques of traction-force microscopy.
了解细胞 - 分子相互作用水平上的趋化作用很重要，因为它在癌症、免疫学和微生物学等领域都有相关性，这里仅举几个例子。本研究量化了微流控装置中趋化作用期间流动对细胞迁移的影响。对装置内的趋化梯度进行了建模，并与实验结果进行了比较。使用趋化因子CXCL8在不同流速下对表达转染的CXCR2趋化因子受体的人HL60早幼粒细胞白血病细胞进行趋化实验。细胞轨迹被分离为x轴和y轴分量。当微通道流速增加时，发现沿x轴的细胞轨迹受到显著影响（p < 0.05）。总迁移距离不受影响。在使用类似的微流控装置进行趋化研究时应考虑这些结果，以便将流动偏差降至最低。利用这种效应来估计细胞在趋化过程中施加的总牵引力可能是可行的，这对于其牵引力低于标准牵引力显微镜检测水平的细胞尤为有价值。
