Nerem R M, Alexander R W, Chappell D C, Medford R M, Varner S E, Taylor W R
Georgia Institute of Technology, Atlanta, Georgia 30332-0363, USA.
Am J Med Sci. 1998 Sep;316(3):169-75. doi: 10.1097/00000441-199809000-00004.
It is now recognized that the mechanical environment of a cell has an influence on its structure and function. For the vascular endothelial cell that resides at the interface of the flowing blood and the underlying vessel wall, there is mounting evidence of the importance of flow and the associated wall shear stress in the regulation of endothelial biology. Not only is it a sensitive regulator of endothelial structure and function, but different flow environments will influence endothelial cell biology differently. Furthermore, there may be an interaction between the chemical environment of a cell and its mechanical environment. This is illustrated by the inhibition by steady laminar shear stress of the cytokine induction of VCAM-1. Results also are presented in which flow studies have been conducted using a co-culture model of the vessel wall. These experiments provide evidence of a quiescent endothelium; however, much more needs to be done to engineer the cell culture environment to make it more physiologic.
现在人们认识到，细胞的力学环境会对其结构和功能产生影响。对于位于流动血液与下方血管壁界面处的血管内皮细胞，越来越多的证据表明血流及相关的壁面剪应力在调节内皮生物学方面具有重要性。它不仅是内皮结构和功能的敏感调节因子，而且不同的流动环境会对内皮细胞生物学产生不同的影响。此外，细胞的化学环境与其力学环境之间可能存在相互作用。这通过稳态层流剪应力对细胞因子诱导的VCAM - 1的抑制作用得到了说明。还展示了使用血管壁共培养模型进行流动研究的结果。这些实验提供了静止内皮的证据；然而，要设计更接近生理状态的细胞培养环境，还有很多工作要做。
