Degré G, Brunet E, Dodge A, Tabeling P
Microfluidics, MEMS, Nanostructure, Ecole Superieure de Physique et Chimie Industrielle, 10 rue Vauquelin, 75231, Paris, France.
Lab Chip. 2005 Jun;5(6):691-4. doi: 10.1039/b501695a. Epub 2005 May 3.
Latex agglutination tests are used for the diagnosis of diseases in man and animals. They are generally simple, cheap, and do not require sophisticated equipment, nor highly specialized skills. In this Technical Note, we put latex agglutination tests in a microfluidic format. The experiment is performed in PDMS (polydimethylsiloxane) microchannels, using streptavidin-coated superparamagnetic beads and a magnetic field. The target molecule is biotinylated protein A. By taking full advantage of the microfluidic conditions (scaling down of the detection volume and controlled action of the shear flow), we achieved an analytical sensitivity of 10 fmol l(-1)(several hundreds of fg ml(-1)) and a fast response (a few minutes) ; the test is also quantitative. Performances of agglutination tests can thus be improved by orders of magnitude by adapting them to a microfluidic format; this comes in addition to the usual advantages offered by this technology (integration, high throughput etc.).
乳胶凝集试验用于人和动物疾病的诊断。它们通常简单、廉价，不需要复杂的设备，也不需要高度专业化的技能。在本技术说明中，我们将乳胶凝集试验制成微流控形式。实验在聚二甲基硅氧烷（PDMS）微通道中进行，使用链霉亲和素包被的超顺磁性微珠和磁场。目标分子是生物素化的蛋白A。通过充分利用微流控条件（检测体积的缩小和剪切流的可控作用），我们实现了10飞摩尔/升（几百飞克/毫升）的分析灵敏度和快速响应（几分钟）；该测试也是定量的。因此，通过将凝集试验调整为微流控形式，其性能可提高几个数量级；这是该技术通常具有的优势（集成、高通量等）之外的优点。
