Centre for Excellence in Omics-Nano Medical Technology Project Development, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
Sensors (Basel). 2009;9(12):10066-79. doi: 10.3390/s91210066. Epub 2009 Dec 10.
An immunoassay performed on a portable microfluidic device was evaluated for the determination of urinary albumin. An increase in absorbance at 500 nm resulting from immunoagglutination was monitored directly on the poly(dimethylsiloxane) (PDMS) microchip using a portable miniature fibre-optic spectrometer. A calibration curve was linear up to 10 mg L(-1) (r(2) = 0.993), with a detection limit of 0.81 mg L(-1) (S/N = 3). The proposed system showed good precision, with relative standard deviations (RSDs) of 5.1%, when evaluated with 10 mg L(-1) albumin (n = 10). Determination of urinary albumin with the proposed system gave results highly similar to those determined by the conventional spectrophotometric method using immunoturbidimetric detection (r(2) = 0.995; n = 15).
一种在便携式微流控装置上进行的免疫测定法，用于测定尿液中的白蛋白。通过使用便携式微型光纤光谱仪直接在聚二甲基硅氧烷（PDMS）微芯片上监测到 500nm 处吸光度的增加，这是由于免疫凝集引起的。校准曲线在线性范围内高达 10mg/L（r(2) = 0.993），检测限为 0.81mg/L（S/N = 3）。当用 10mg/L 白蛋白（n = 10）评估时，所提出的系统显示出良好的精密度，相对标准偏差（RSD）为 5.1%。用所提出的系统测定尿白蛋白的结果与传统的免疫比浊检测分光光度法非常相似（r(2) = 0.995；n = 15）。
