Caccamo Nadia, Meraviglia Serena, Ferlazzo Viviana, Angelini Daniela, Borsellino Giovanna, Poccia Fabrizio, Battistini Luca, Dieli Francesco, Salerno Alfredo
Dipartimento di Biopatologia e Metodologie Biomediche, Università di Palermo, Palermo, Italy.
Eur J Immunol. 2005 Jun;35(6):1764-72. doi: 10.1002/eji.200525983.
We have compared four human subsets of Vgamma9Vdelta2 T cells, naive (T(naive), CD45RA(+)CD27(+)), central memory (T(CM), CD45RA(-)CD27(+)), effector memory (T(EM), CD45RA(-)CD27(-)) and terminally differentiated (T(EMRA), CD45RA(+)CD27(-)), for their capacity to proliferate and differentiate in response to antigen or homeostatic cytokines. Cytokine responsiveness and IL-15R expression were low in T(naive) cells and progressively increased from T(CM) to T(EM) and T(EMRA) cells. In contrast, the capacity to expand in response to antigen or cytokine stimulation showed a reciprocal pattern and was associated with resistance to cell death and Bcl-2 expression. Whereas antigen-stimulated cells acquired a T(CM) or T(EM) phenotype, IL-15-stimulated cells maintained their phenotype, with the exception of T(CM) cells, which expressed CD27 and CD45RA in various combinations. These results, together with ex vivo bromodeoxyuridine incorporation experiments, show that human Vgamma9Vdelta2 memory T cells have different proliferation and differentiation potentials in vitro and in vivo and that T(EMRA) cells are generated from the T(CM) subset upon homeostatic proliferation in the absence of antigen.
我们比较了Vγ9Vδ2 T细胞的四个人类亚群，即初始型（T(naive)，CD45RA(+)CD27(+)）、中央记忆型（T(CM)，CD45RA(-)CD27(+)）、效应记忆型（T(EM)，CD45RA(-)CD27(-)）和终末分化型（T(EMRA)，CD45RA(+)CD27(-)），观察它们在对抗抗原或稳态细胞因子时的增殖和分化能力。T(naive)细胞的细胞因子反应性和IL-15R表达较低，从T(CM)细胞到T(EM)细胞再到T(EMRA)细胞逐渐增加。相反，对抗原或细胞因子刺激的扩增能力呈现相反的模式，并且与细胞死亡抗性和Bcl-2表达相关。抗原刺激的细胞获得T(CM)或T(EM)表型，而IL-15刺激的细胞保持其表型，但T(CM)细胞除外，其以各种组合表达CD27和CD45RA。这些结果与体外溴脱氧尿苷掺入实验一起表明，人类Vγ9Vδ2记忆T细胞在体外和体内具有不同的增殖和分化潜能，并且在没有抗原的情况下，T(EMRA)细胞是在稳态增殖过程中从T(CM)亚群产生的。
