Mayers Carl N, Lee Kian-Chung, Moore Catherine A, Wong Sek-Man, Carr John P
Plant Sciences Department, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.
Mol Plant Microbe Interact. 2005 May;18(5):428-34. doi: 10.1094/MPMI-18-0428.
Salicylic acid (SA)-induced resistance to Cucumber mosaic virus (CMV) in tobacco (Nicotiana tabacum) results from inhibition of systemic virus movement and is induced via a signal transduction pathway that also can be triggered by antimycin A, an inducer of the mitochondrial enzyme alternative oxidase (AOX). In Arabidopsis thaliana, inhibition of CMV systemic movement also is induced by SA and antimycin A. These results indicate that the mechanisms underlying induced resistance to CMV in tobacco and A. thaliana are very similar. In contrast to the situation in tobacco and A. thaliana, in squash (Cucurbita pepo), SA-induced resistance to CMV results from inhibited virus accumulation in directly inoculated tissue, most likely through inhibition of cell-to-cell movement. Furthermore, neither of the AOX inducers antimycin A or KCN induced resistance to CMV in squash. Additionally, AOX inhibitors that compromise SA-induced resistance to CMV in tobacco did not inhibit SA-induced resistance to the virus in squash. The results show that different host species may use significantly different approaches to resist infection by the same virus. These findings also imply that caution is required when attempting to apply findings on plant-virus interactions from model systems to a wider range of host species.
水杨酸（SA）诱导烟草（Nicotiana tabacum）对黄瓜花叶病毒（CMV）产生抗性，这是通过抑制病毒的系统移动实现的，并且是经由一条信号转导途径诱导产生的，该途径也可被抗霉素A触发，抗霉素A是线粒体酶交替氧化酶（AOX）的诱导剂。在拟南芥（Arabidopsis thaliana）中，SA和抗霉素A也会诱导对CMV系统移动的抑制。这些结果表明，烟草和拟南芥中诱导产生对CMV抗性的机制非常相似。与烟草和拟南芥的情况不同，在南瓜（Cucurbita pepo）中，SA诱导的对CMV的抗性源于直接接种组织中病毒积累的抑制，很可能是通过抑制细胞间移动实现的。此外，AOX诱导剂抗霉素A或KCN均未在南瓜中诱导对CMV的抗性。另外，在烟草中损害SA诱导的对CMV抗性的AOX抑制剂，并未抑制SA在南瓜中诱导的对该病毒的抗性。结果表明，不同的宿主物种可能采用显著不同的方法来抵抗同一种病毒的感染。这些发现还意味着，在试图将模型系统中关于植物 - 病毒相互作用的研究结果应用于更广泛的宿主物种时，需要谨慎行事。
