Abdelkhalek Ahmed, Király Lóránt, Al-Mansori Al-Naji A, Younes Hosny A, Zeid Ahmed, Elsharkawy Mohsen Mohamed, Behiry Said I
Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, Alexandria 21934, Egypt.
Centre for Agricultural Research, Plant Protection Institute, ELKH, 15 Herman Ottó Str., H-1022 Budapest, Hungary.
Plants (Basel). 2022 Jul 23;11(15):1908. doi: 10.3390/plants11151908.
The current study focuses on the effects of (CMV) infection on phytochemical changes and pathogenesis- and phenylpropanoid pathway-associated gene activities in squash ( L.) plants during a time course of 2 to 12 days post inoculation (dpi). The identity of the CMV isolate was confirmed by DAS-ELISA, TEM, and coat protein gene sequence. The CMV infection initially boosts and then suppresses transcript levels of the defense-related genes , and during the investigated time course compared to controls. The expression profile during the time-course study indicated that early, transient induction of occurs during CMV infection, while CMV induced the expression of in systemically infected squash tissues at all time points and suppressed the expression of and at 8-12 dpi. transcript levels fluctuated between up- and down-regulation, but by 12 dpi, expression reached its peak. The HPLC and GC-MS analyses of CMV-infected squash extracts revealed that different phenolic, flavonoid, and fatty acid compounds could be induced or suppressed upon CMV infection. In particular, CMV could suppress the synthesis of most phenolic compounds, specifically chlorogenic acid, possibly leading to the virus's rapid spread.
本研究聚焦于黄瓜花叶病毒（CMV）感染对西葫芦（西葫芦属）植株接种后2至12天病程中植物化学变化以及与发病机制和苯丙烷途径相关的基因活性的影响。通过双抗夹心酶联免疫吸附测定（DAS-ELISA）、透射电子显微镜（TEM）和外壳蛋白基因序列鉴定了CMV分离株。与对照相比，在研究的病程中，CMV感染最初会促进然后抑制防御相关基因、和的转录水平。病程研究期间的表达谱表明，CMV感染期间会早期短暂诱导，而CMV在所有时间点均诱导系统感染的西葫芦组织中的表达，并在接种后8至12天抑制和的表达。的转录水平在上调和下调之间波动，但到接种后12天，的表达达到峰值。对感染CMV的西葫芦提取物进行的高效液相色谱（HPLC）和气相色谱-质谱联用（GC-MS）分析表明，CMV感染可诱导或抑制不同的酚类、黄酮类和脂肪酸化合物。特别是，CMV可抑制大多数酚类化合物的合成，尤其是绿原酸，这可能导致病毒的快速传播。
