Gharib H A, Mandour A M
Timber Trees and Forestry Research Department, Horticulture Research Institute, Agricultural Research Center, 9 St. Al-Gamaa, Giza, Egypt.
Plant Virus and Phytoplasma Research Department, Plant Pathology Research Institute, Agricultural Research Center, 9 St. Al-Gamaa, Giza, Egypt.
Sci Rep. 2022 Dec 23;12(1):22194. doi: 10.1038/s41598-022-24786-2.
Capsicum annuum is one of the main vegetable crops for the local market and exportation in Egypt. In this concern, pepper mild mottle virus (PMMoV) infection caused a significant decrease in Capsicum sp. leading to large economic losses. An isolate of PMMoV was obtained from naturally infected pepper plants, exhibiting different patterns of mottling, leaf distortion, yellowing, and stunting of leaves. The virus was identified. The molecular detection of PMMoV was done using RT-PCR with specific primers designed for coat protein genes. An RT-PCR product (474) bp of the coat protein gene of (PMMoV) was cloned. The target of the investigation was the effect of spring and autumn ethanol extracts of Populus nigra leaves on C. annuum seedling growth and infected C. annuum with (PMMoV) under greenhouse conditions. The experimental data showed that treated spring leaf extract of P. nigra enhanced infected C. annuum seedling growth parameters and fruit quality compared to uninfected seedlings. P. nigra spring leaf extract containing some allo-chemicals had a negative effect on uninfected seedlings. P. nigra autumn leaf extract significantly improved the growth and fruit quality of infected C. annuum seedlings compared to the control.
辣椒是埃及当地市场和出口的主要蔬菜作物之一。在这方面，辣椒轻斑驳病毒（PMMoV）感染导致辣椒属植物显著减产，造成巨大经济损失。从自然感染的辣椒植株中获得了一株PMMoV分离株，该植株表现出不同的斑驳、叶片扭曲、黄化和叶片发育迟缓模式。该病毒得到了鉴定。使用针对外壳蛋白基因设计的特异性引物，通过RT-PCR对PMMoV进行分子检测。克隆了PMMoV外壳蛋白基因的一个RT-PCR产物（474）bp。研究的目的是在温室条件下，研究黑杨树叶春季和秋季乙醇提取物对辣椒幼苗生长以及感染PMMoV的辣椒的影响。实验数据表明，与未感染的幼苗相比，经黑杨树叶春季叶提取物处理的感染辣椒幼苗的生长参数和果实品质有所提高。含有一些化感物质的黑杨树叶春季叶提取物对未感染的幼苗有负面影响。与对照相比，黑杨树叶秋季叶提取物显著改善了感染辣椒幼苗的生长和果实品质。
