Wallace D M, Magnuson D J, Gray T S
Department of Cell Biology, Neurobiology, and Anatomy, Loyola Stritch School of Medicine, Maywood, IL 60153.
Brain Res Bull. 1992 Mar;28(3):447-54. doi: 10.1016/0361-9230(92)90046-z.
The distribution of amygdaloid axons in the various brainstem dopaminergic, noradrenergic, and adrenergic cell groups was examined. This was accomplished by means of the Phaseolus vulgaris leucoagglutinin lectin (PHA-L) anterograde tracing technique combined with glucose-oxidase immunocytochemistry to catecholamine markers (i.e., tyrosine hydroxylase, dopamine beta hydroxylase, and phenylethanolamine N-methyltransferase). Injections of PHA-L in the medial part of the central amygdaloid nucleus resulted in axonal and terminal labeling in most catecholamine cell groups in the brainstem. Amygdaloid terminals appeared to contract catecholaminergic cells in several brainstem regions. The most heavily innervated catecholaminergic cells were the A9 (lateral) and A8 dopaminergic cell groups and the C2/A2 adrenergic/noradrenergic cell groups in the nucleus of the solitary tract. The medial part of the A9 and adjacent A10 dopaminergic cell groups was moderately innervated. A moderate innervation by amygdaloid terminals was observed on rostral locus coeruleus noradrenergic cells (A6 rostral) and adrenergic cells of the rostral ventrolateral medulla (C1). Noradrenergic cells of the A5, main body of the locus coeruleus (A6), A7, and subcoeruleus were sparsely innervated. Amygdaloid axons were not observed on noradrenergic neurons of the A4 cell group, area postrema, and A1 cells of the ventrolateral medulla. The results demonstrate that the amygdala primarily innervates the dopaminergic cells of midbrain (i.e., A8 and lateral A9 cells) and the adrenergic cells (C2) and noradrenergic (A2) cells in the nucleus of the solitary tract. The possible functional significance of amygdaloid innervation of catecholaminergic cells is discussed.
研究了杏仁核轴突在各种脑干多巴胺能、去甲肾上腺素能和肾上腺素能细胞群中的分布。这是通过菜豆白细胞凝集素（PHA-L）顺行追踪技术与针对儿茶酚胺标志物（即酪氨酸羟化酶、多巴胺β羟化酶和苯乙醇胺N-甲基转移酶）的葡萄糖氧化酶免疫细胞化学相结合来完成的。将PHA-L注射到中央杏仁核核的内侧部分，导致脑干中大多数儿茶酚胺细胞群出现轴突和终末标记。杏仁核终末似乎在几个脑干区域与儿茶酚胺能细胞形成突触联系。接受最密集神经支配的儿茶酚胺能细胞是A9（外侧）和A8多巴胺能细胞群以及孤束核中的C2/A2肾上腺素能/去甲肾上腺素能细胞群。A9的内侧部分和相邻的A10多巴胺能细胞群接受中等程度的神经支配。在蓝斑嘴侧去甲肾上腺素能细胞（A6嘴侧）和嘴侧腹外侧延髓的肾上腺素能细胞（C1）上观察到杏仁核终末的中等程度神经支配。A5、蓝斑主体（A6）、A7和蓝斑下核的去甲肾上腺素能细胞接受稀疏的神经支配。在A4细胞群、最后区的去甲肾上腺素能神经元以及腹外侧延髓的A1细胞上未观察到杏仁核轴突。结果表明，杏仁核主要支配中脑的多巴胺能细胞（即A8和外侧A9细胞）以及孤束核中的肾上腺素能细胞（C2）和去甲肾上腺素能细胞（A2）。讨论了杏仁核对儿茶酚胺能细胞神经支配的可能功能意义。
