Wiengcharoen Jitbanjong Toomphong, Chiabchalard Rachatawan, Sukthana Yaowalark
Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok.
Southeast Asian J Trop Med Public Health. 2004 Dec;35(4):792-5.
The goal of diagnosing congenital toxoplasmosis is early detection of maternofetal transmission, for early treatment to prevent unwanted sequelae. Polymerase chain reaction (PCR) is a method used recently for detecting toxoplasmosis during pregnancy. Amniotic fluid is a the clinical specimen used, since it provides a rapid, simple and safe method to obtain accurate results. The advantages of the PCR technique are high sensitivity, specificity and positive predictive value compared with other laboratory methods. To determine the sensitivity, specificity and lower detection limits in our laboratory, amplification of the B1 gene by nested PCR was performed on Toxoplasma gondii tachyzoites added to animal amniotic fluid samples. From 48 samples, our technique detected T. gondii in 30 out of 41 positive samples, and gave negative results for all the negative samples. The sensitivity for this nested PCR was 73%, the specificity was 100%, and the efficiency of the test was 77.1%. The nested PCR technique is recommended as a diagnostic method for detecting T. gondii in suspected congenital toxoplasmosis animals.
诊断先天性弓形虫病的目的是早期检测母婴传播，以便早期治疗以预防不良后遗症。聚合酶链反应（PCR）是最近用于孕期检测弓形虫病的一种方法。羊水是所使用的临床标本，因为它提供了一种快速、简单且安全的方法来获得准确结果。与其他实验室方法相比，PCR技术的优点是具有高灵敏度、特异性和阳性预测值。为了确定我们实验室的灵敏度、特异性和较低检测限，对添加到动物羊水样本中的弓形虫速殖子进行巢式PCR扩增B1基因。在48个样本中，我们的技术在41个阳性样本中的30个中检测到了弓形虫，并且对所有阴性样本均给出了阴性结果。这种巢式PCR的灵敏度为73%，特异性为100%，检测效率为77.1%。巢式PCR技术被推荐作为检测疑似先天性弓形虫病动物中弓形虫的诊断方法。
