Cetin Meral, Pinarbasi Ergun, Percin Ferda E, Akgün Egemen, Percin Sibel, Pinarbasi Hatice, Gurlek Fatma, Cetin Ali
Department of Obstetrics and Gynecology, Cumhuriyet University School of Medicine, Sivas, Turkey.
J Obstet Gynaecol Res. 2005 Jun;31(3):236-41. doi: 10.1111/j.1447-0756.2005.00281.x.
There is substantial evidence that genetic factors play a role in pre-eclampsia. The aim of this study was to determine whether genetic variability in the encoding of genes for glutathione S-transferase M1 (GSTM1) and glutathione S-transferase T1 (GSTT1) contributes to individual differences in susceptibility to pre-eclampsia, eclampsia, or hemolysis, elevated liver enzymes, and low platelets (HELLP syndrome).
A total of 221 women with pre-eclampsia, eclampsia and HELLP syndrome and 147 healthy female controls were genotyped for GSTM1 and GSTT1 polymorphisms by polymerase chain reaction (PCR). Statistical evaluation of differences in polymorphic rates was carried out using chi(2) analysis.
This study included 140 pre-eclamptic, 33 eclamptic and 48 HELLP syndrome cases and 147 healthy controls. The frequencies for the GSTM1 null genotype were 58%, 45%, and 60% for pre-eclampsia, eclampsia, and HELLP syndrome, respectively, and in controls it was 55%. The distribution of the GSTT1 null genotype was 22%, 21%, and 27% for pre-eclampsia, eclampsia, and HELLP syndrome, respectively, and in controls it was 22%. There was no significant association between GSTM1 and GSTT1 polymorphisms and pre-eclampsia, eclampsia, and HELLP syndrome.
Our data do not support a role for polymorphisms of the GSTM1 and GSTT1 genes in the pathogenesis of pre-eclampsia, eclampsia and HELLP syndrome.
有大量证据表明遗传因素在子痫前期中起作用。本研究的目的是确定谷胱甘肽S-转移酶M1（GSTM1）和谷胱甘肽S-转移酶T1（GSTT1）基因编码中的遗传变异性是否导致个体患子痫前期、子痫或溶血、肝酶升高及血小板减少（HELLP综合征）易感性的差异。
采用聚合酶链反应（PCR）对221例患有子痫前期、子痫和HELLP综合征的女性及147例健康女性对照进行GSTM1和GSTT1基因多态性基因分型。使用卡方分析对多态性率的差异进行统计学评估。
本研究纳入了140例子痫前期患者、33例子痫患者和48例HELLP综合征患者以及147例健康对照。子痫前期、子痫和HELLP综合征患者中GSTM1无效基因型的频率分别为58%、45%和60%，而对照组为55%。GSTT1无效基因型在子痫前期、子痫和HELLP综合征患者中的分布分别为22%、21%和27%，对照组为22%。GSTM1和GSTT1基因多态性与子痫前期、子痫和HELLP综合征之间无显著关联。
我们的数据不支持GSTM1和GSTT1基因多态性在子痫前期、子痫和HELLP综合征发病机制中起作用。
