Department of Biology, College of Sciences Shiraz University, 71454 Shiraz, Iran.
Mol Biol Rep. 2012 Sep;39(9):8995-8. doi: 10.1007/s11033-012-1769-7. Epub 2012 Jun 24.
Preeclampsia (PE) is a complex disorder affected by genetic and environmental factors. Although the exact genes involved in development of PE are still not fully discovered, an important role for oxidative stress in its pathogenesis is accepted. In the present study, the association between the functional genetic polymorphisms in codons 32, 42 and nucleotide -1002 of glutathione S-transferases Z1 (GSTZ1) and susceptibility to PE was investigated. The present case-control study was performed on 151 preeclapmtic patients, and a total of 200 normal pregnant women, as a control group. The healthy control group was frequency matched with the age of the preeclamptic patients. Control subjects had no history of previous pregnancies with PE. Genotypes were determined by PCR-RFLP assay. There was no significant association between G-1002A and Glu32Lys polymorphisms of GSTZ1 with PE risk. The variant allele of Gly42Arg polymorphism decreased the risk of PE (OR = 0.24, 95 % CI 0.08-0.73, P = 0.012). The haplotype of "-1002A, 32Lys, 42Arg" (having three variant alleles) versus to the other haplotypes significantly decreased among PE patients compared to the control group (5.0 vs. 0.9 percent among control and PE patient groups, respectively; χ(2) = 9.328, df = 1, P = 0.002). The present results indicate that the haplotype of "-1002A, 32Lys, 42Arg" (containing three variant alleles) of GSTZ1 have protective effect compared to the other haplotypes.
子痫前期（PE）是一种受遗传和环境因素影响的复杂疾病。尽管涉及 PE 发展的确切基因尚未完全发现，但氧化应激在其发病机制中的重要作用已被接受。在本研究中，研究了谷胱甘肽 S-转移酶 Z1（GSTZ1）密码子 32、42 和核苷酸-1002 中的功能遗传多态性与 PE 易感性之间的关系。本病例对照研究共纳入 151 例子痫前期患者和 200 名正常妊娠妇女作为对照组。健康对照组与子痫前期患者的年龄相匹配。对照组无 PE 史。通过 PCR-RFLP 分析确定基因型。GSTZ1 的 G-1002A 和 Glu32Lys 多态性与 PE 风险之间无显著相关性。Gly42Arg 多态性的变异等位基因降低了 PE 的风险（OR=0.24，95%CI 0.08-0.73，P=0.012）。与其他单倍型相比，“-1002A、32Lys、42Arg”的单倍型（有三个变异等位基因）在 PE 患者中明显减少（5.0%比对照组和 PE 患者组分别为 0.9%；χ(2)=9.328，df=1，P=0.002）。本研究结果表明，与其他单倍型相比，GSTZ1 的“-1002A、32Lys、42Arg”（含有三个变异等位基因）单倍型具有保护作用。
