Mendis Eresha, Rajapakse Niranjan, Byun Hee-Guk, Kim Se-Kwon
Department of Chemistry, Pukyong National University, Busan 608-737, South Korea.
Life Sci. 2005 Sep 9;77(17):2166-78. doi: 10.1016/j.lfs.2005.03.016.
Peptides derived from tryptic hydrolysate of jumbo squid (Dosidicus gigas) skin gelatin were assessed for their antioxidant properties in different in vitro assay systems. The hydrolysate itself exhibited a strong lipid peroxidation inhibition and it was much higher than that of natural antioxidant, alpha-tocopherol. In addition, it could scavenge highly active free radicals in oxidative systems, in the order of hydroxyl and carbon-centered radicals. Two representative peptides with comparatively higher antioxidant potency were purified and characterized as Phe-Asp-Ser-Gly-Pro-Ala-Gly-Val-Leu (880.18 Da) and Asn-Gly-Pro-Leu-Gln-Ala-Gly-Gln-Pro-Gly-Glu-Arg (1241.59 Da). Furthermore, viability of radical-mediated oxidation-induced human lung fibroblasts was enhanced following the treatment of two peptides. However it did not exhibit substantial ion chelation, and we presumed that the observed radical scavenging potency of these peptides play a vital role for their strong antioxidant activity. Based on our results we suggest that hydrophobic amino acids present in peptide sequences contributed greatly for observed antioxidant activities.
对源自巨型鱿鱼（茎柔鱼）皮肤明胶胰蛋白酶水解产物的肽段在不同体外分析系统中的抗氧化特性进行了评估。水解产物本身表现出很强的脂质过氧化抑制作用，且远高于天然抗氧化剂α-生育酚。此外，它能够清除氧化系统中高活性自由基，清除能力顺序为羟基自由基和碳中心自由基。纯化并鉴定了两种具有相对较高抗氧化能力的代表性肽段，分别为Phe-Asp-Ser-Gly-Pro-Ala-Gly-Val-Leu（880.18 Da）和Asn-Gly-Pro-Leu-Gln-Ala-Gly-Gln-Pro-Gly-Glu-Arg（1241.59 Da）。此外，用这两种肽处理后，自由基介导的氧化诱导的人肺成纤维细胞的活力增强。然而，它并未表现出显著的离子螯合作用，我们推测这些肽段所观察到的自由基清除能力对其强大的抗氧化活性起着至关重要的作用。基于我们的结果，我们认为肽序列中存在的疏水氨基酸对所观察到的抗氧化活性有很大贡献。
