Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea.
Int J Biol Macromol. 2011 Dec 1;49(5):1110-6. doi: 10.1016/j.ijbiomac.2011.09.009. Epub 2011 Sep 16.
Potent antioxidative peptides were purified from Pacific cod (Gadus macrocephalus) skin gelatin using alcalase, neutrase, papain, trypsin, pepsin, and α-chymotrypsin. Among them, the papain hydrolysate exhibited the highest antioxidant activity. Therefore, it was further purified and obtained two peptides with amino acid sequences of Thr-Cys-Ser-Pro (388 Da) and Thr-Gly-Gly-Gly-Asn-Val (485.5 Da). The antioxidant activity of the purified peptides was performed by electron spin resonance technique. Moreover, their intracellular free radical scavenging activity using 2',7'-dichlorofluorescin diacetate and the protective effect against oxidation-induced DNA damage were evaluated in mouse macrophages (RAW 264.7 cells). Furthermore, both peptides have shown potential angiotensin-I converting enzyme inhibitory effect. The present study demonstrated that the peptides derived from Pacific cod (G. macrocephalus) skin gelatin could be used in the food industry as functional ingredients with potent antioxidative and antihypertensive benefits.
采用碱性蛋白酶、中性蛋白酶、木瓜蛋白酶、胰蛋白酶、胃蛋白酶和α-糜蛋白酶从太平洋鳕鱼（大头鳕）皮明胶中纯化出具有强抗氧化活性的肽。其中，木瓜蛋白酶水解物的抗氧化活性最高。因此，进一步对其进行纯化，得到两个氨基酸序列分别为 Thr-Cys-Ser-Pro（388 Da）和 Thr-Gly-Gly-Gly-Asn-Val（485.5 Da）的肽。采用电子自旋共振技术研究了纯化肽的抗氧化活性，并通过 2'，7'-二氯荧光素二乙酸酯评价了其在小鼠巨噬细胞（RAW 264.7 细胞）内清除自由基的活性和对氧化诱导的 DNA 损伤的保护作用。此外，两种肽均表现出潜在的血管紧张素转化酶抑制作用。本研究表明，来源于太平洋鳕鱼（大头鳕）皮明胶的肽可用作食品工业中的功能性成分，具有较强的抗氧化和降血压作用。
