Fleury Agnès, Beltran Constantino, Ferrer Elizabeth, Garate Terésa, Harrison Leslie J S, Parkhouse R Michael E, Garcia Esperanza, Fragoso Gladis, Costa-Cruz Julia, Biondi Germano, Agapejev Svetlana, Sciutto Edda
Instituto Nacional de Neurología y Neurocirugía, México, DF México.
Trop Med Int Health. 2003 Dec;8(12):1124-30. doi: 10.1046/j.1360-2276.2003.01132.x.
We tested the possible diagnostic utility of five Taenia saginata oncosphere-derived synthetic peptides in T. solium neurocysticercosis (NC). The five peptides correspond to protein sequences with high antigenic indexes that were cloned from a T. saginata oncosphere cDNA library. The test samples consisted of cerebrospinal fluid (CSF) samples randomly collected from patients referred from Mexican and Brazilian neurological institutes. Indirect enzyme-linked immunosorbent assays (ELISA) were carried out with the peptides either unconjugated or coupled to carrier proteins, and were compared with results obtained using T. solium cyst fluid as a positive control. For active inflammatory NC, the higher sensibility (93%) and specificity (85%) was obtained with peptides HP6-2 and Ts45W-1, respectively, coupled to ovalbumin, in both Mexican and Brazilian patients. Examining the results of the individual peptide assays in combination, in some instances, improved the sensitivity to 100%.
我们测试了五种牛带绦虫六钩蚴衍生的合成肽在猪带绦虫神经囊尾蚴病（NC）中的潜在诊断效用。这五种肽对应于从牛带绦虫六钩蚴cDNA文库中克隆出的具有高抗原指数的蛋白质序列。测试样本包括从墨西哥和巴西神经学研究所转诊患者中随机收集的脑脊液（CSF）样本。使用未偶联或偶联载体蛋白的肽进行间接酶联免疫吸附测定（ELISA），并与使用猪带绦虫囊液作为阳性对照所获得的结果进行比较。对于活动性炎性NC，在墨西哥和巴西患者中，分别与卵清蛋白偶联的肽HP6-2和Ts45W-1的敏感性（93%）和特异性（85%）更高。在某些情况下，综合检查各个肽检测的结果可将敏感性提高到100%。
