Zschöck M, Kloppert Bärbel, Wolter W, Hamann H P, Lämmler Ch
Hessisches Landeslabor, Abteilung II (Veterinärmedizin), Marburger Str. 54, 35396 Giessen, Germany.
Vet Microbiol. 2005 Jul 1;108(3-4):243-9. doi: 10.1016/j.vetmic.2005.02.012.
PCR detection of the genes encoding the newly described staphylococcal enterotoxins (SE) SEG, SEH, SEI and SEJ was carried out for 104 randomly selected Staphylococcus aureus field strains isolated from cases of bovine mastitis. Sixty-one (58.7%) isolates were positive for one or more of these novel enterotoxin genes. Thirty-six field strains were classified as carrier of seg, 22 of sei gene and 23 were positive for sej gene. None of the 104 investigated ruminant S. aureus strains carried the seh gene. Thirty-seven of these S. aureus strains showed a combination of genes encoding enterotoxin types SEA to SEE or toxic shock syndrome toxin 1 (TSST 1). Thirteen cultures harboured only one, 28 two, 12 three and 8 four enterotoxin genes. Among the 61 S. aureus field strains 14 (23.0%) were positive for the genes encoding SEJ and SED and 10 (16.4%) isolates for those encoding SEG and SEI. Isolates harbouring the sed/sej genes were further characterized by macrorestriction analysis and pulsed-field-gelelectrophoresis (Pfge). Macrorestriction analysis revealed six patterns. Nine of these14 S. aureus isolates (64.3%) exhibited two patterns with a high degree of relationship (>80%).
对从牛乳腺炎病例中随机选取的104株金黄色葡萄球菌田间菌株进行聚合酶链反应（PCR）检测，以检测编码新描述的葡萄球菌肠毒素（SE）SEG、SEH、SEI和SEJ的基因。61株（58.7%）分离株对这些新型肠毒素基因中的一种或多种呈阳性。36株田间菌株被分类为seg携带者，22株为sei基因携带者，23株sej基因呈阳性。104株被调查的反刍动物金黄色葡萄球菌菌株均未携带seh基因。其中37株金黄色葡萄球菌菌株显示出编码肠毒素SEA至SEE或中毒性休克综合征毒素1（TSST 1）的基因组合。13个培养物仅含有一种、28个含有两种、12个含有三种和8个含有四种肠毒素基因。在61株金黄色葡萄球菌田间菌株中，14株（23.0%）编码SEJ和SED的基因呈阳性，10株（16.4%）分离株编码SEG和SEI的基因呈阳性。通过宏观限制性分析和脉冲场凝胶电泳（Pfge）对携带sed/sej基因的分离株进行进一步表征。宏观限制性分析揭示了六种模式。这14株金黄色葡萄球菌分离株中的9株（64.3%）表现出两种高度相关（>80%）的模式。
