Pumipuntu Natapol
One Health Research Unit, Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, Thailand.
Vet World. 2019 Dec;12(12):1940-1944. doi: 10.14202/vetworld.2019.1940-1944. Epub 2019 Dec 12.
is an emerging species of the complex. It has usually been misidentified as by conventional methods and its characteristics. is potentially emerging in both humans and animals with an increasing global distribution. This study aimed to differentiate and identify from collected and isolated from milk samples of subclinical bovine mastitis cases in Maha Sarakham Province, Northeastern Thailand.
Forty-two isolates of were studied from 132 individual milk samples collected from subclinical bovine mastitis cases of 15 dairy farms in three districts of Maha Sarakham, Thailand. The identification was confirmed by conventional and immune-agglutination methods. Fifteen representative isolates which were suspected as being were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).
The result from MALDI-TOF MS confirmed that seven from 15 isolates were and eight isolates were .
This study indicated that MALDI-TOF MS used as an identification and classification method could accurately differentiate the novel species, , from the complex which is usually misdiagnosed. In addition, the identification of seems to be very limited in technical difficulty despite the fact that it may be the important causative pathogen in bovine mastitis as well as a pathogenic bacterium in food and milk. Therefore, it is essential for both bovine medicine and veterinary public health to emphasize and recognize this bacterial pathogen as an emerging disease of staphylococcal bacteria that there is a need for further study of infections.
[具体物种名称1]是[所属菌群名称]菌群中的一个新出现的物种。传统方法通常会根据其特征将其误鉴定为[具体物种名称2]。[具体物种名称1]在人类和动物中都有潜在出现的情况，且在全球的分布范围不断扩大。本研究旨在对从泰国东北部玛哈沙拉堪府亚临床型牛乳腺炎病例的牛奶样本中收集和分离出的[具体物种名称1]与[具体物种名称2]进行区分和鉴定。
从泰国玛哈沙拉堪三个区15个奶牛场的亚临床型牛乳腺炎病例采集的132份个体牛奶样本中，研究了42株[具体物种名称1]分离株。通过传统方法和免疫凝集法进行鉴定。对15株疑似为[具体物种名称1]的代表性分离株采用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）进行分析。
MALDI-TOF MS结果证实，15株中有7株为[具体物种名称1]，8株为[具体物种名称2]。
本研究表明，MALDI-TOF MS作为一种鉴定和分类方法，能够准确区分新物种[具体物种名称1]与通常被误诊的[所属菌群名称]菌群。此外，尽管[具体物种名称1]可能是牛乳腺炎的重要致病病原体以及食品和牛奶中的病原菌，但对其进行鉴定的技术难度似乎非常有限。因此，对于牛医学和兽医公共卫生而言，强调并认识到这种细菌病原体是葡萄球菌属细菌的一种新出现疾病，有必要对[具体物种名称1]感染进行进一步研究，这一点至关重要。
