Hininger Isabelle, Waters Robert, Osman Mireille, Garrel Catherine, Fernholz Karen, Roussel Anne Marie, Anderson Richard A
Laboratoire NVMC (Nutrition, Vieillissement, Maladies Cardiovasculaires), EA 3746, J. Fourier University, Domaine de la Merci, 38700 La Tronche, France.
Free Radic Biol Med. 2005 Jun 15;38(12):1565-70. doi: 10.1016/j.freeradbiomed.2005.02.016. Epub 2005 Mar 17.
Chelation therapy is thought to not only remove contaminating metals but also to decrease free radical production. EDTA chelation therapy, containing high doses of vitamin C as an antioxidant, is often used in the treatment of diseases such as diabetes and cardiovascular diseases but the effectiveness of this treatment may be variable and its efficacy has not been demonstrated conclusively. The objective of this work was to determine if the vitamin C added to standard chelation therapy cocktails was prooxidant. We administered a standard EDTA cocktail solution with or without 5 g of sodium ascorbate. One hour following the standard chelation therapy, there were highly significant prooxidant effects on lipids, proteins, and DNA associated with decreased activities of RBC glutathione peroxidase and superoxide dismutase while in the absence of sodium ascorbate, there were no acute signs of oxidative damage. After 16 sessions of standard chelation therapy, the acute prooxidant effects of vitamin C remained, but, even in the absence of nutrient supplements, there were beneficial long-term antioxidant effects of chelation therapy and plasma peroxide levels decreased. In conclusion, multiple sessions of EDTA chelation therapy protect lipids against oxidative damage. However, standard high amounts of vitamin C added to EDTA chelation solutions also display short term prooxidant effects. The added benefits of lower levels of vitamin C in chelation therapy need to be documented.
螯合疗法被认为不仅可以去除污染金属，还能减少自由基的产生。含有高剂量维生素C作为抗氧化剂的乙二胺四乙酸（EDTA）螯合疗法常用于治疗糖尿病和心血管疾病等，但这种治疗方法的效果可能因人而异，其疗效尚未得到确凿证实。这项研究的目的是确定添加到标准螯合疗法配方中的维生素C是否具有促氧化作用。我们给予了含或不含5克抗坏血酸钠的标准EDTA混合溶液。在标准螯合疗法1小时后，对脂质、蛋白质和DNA产生了高度显著的促氧化作用，同时红细胞谷胱甘肽过氧化物酶和超氧化物歧化酶的活性降低；而在没有抗坏血酸钠的情况下，没有氧化损伤的急性迹象。经过16次标准螯合疗法后，维生素C的急性促氧化作用仍然存在，但即使在没有营养补充剂的情况下，螯合疗法也产生了有益的长期抗氧化作用，血浆过氧化物水平降低。总之，多次EDTA螯合疗法可保护脂质免受氧化损伤。然而，添加到EDTA螯合溶液中的标准高剂量维生素C也显示出短期促氧化作用。螯合疗法中较低水平维生素C的额外益处需要进一步研究证实。
