Woegerbauer Markus, Lagler Heimo, Graninger Wolfgang, Burgmann Heinz
Department of Infectious Diseases, Department of Internal Medicine I, University of Vienna and Birkmayer Laboratories, Department of Research and Development, Schwarzspanierstrasse 15, A-1090 Vienna, Austria.
Antimicrob Agents Chemother. 2005 Jun;49(6):2490-4. doi: 10.1128/AAC.49.6.2490-2494.2005.
Fragments of erm(E2), otrA, and aph(6) shorter than 400 bp and producer strain-specific rRNA genes were amplified from various antibiotics. The amount of genetic material and the sizes of amplicons recovered from murine feces after oral administration of a beta-lactamase-encoding plasmid indicated substantial DNA degradation in the mammalian gastrointestinal tract. These observations imply that antibiotics are no major source for horizontal resistance gene transfer in clinical settings.
从各种抗生素中扩增出长度小于400 bp的erm(E2)、otrA和aph(6)片段以及产毒株特异性rRNA基因。口服携带β-内酰胺酶编码质粒后从小鼠粪便中回收的遗传物质数量和扩增子大小表明,哺乳动物胃肠道中存在大量DNA降解。这些观察结果表明,在临床环境中,抗生素并非水平耐药基因转移的主要来源。