Gutarra Melissa L E, Cavalcanti Elisa D C, Castilho Leda R, Freire Denise M G, Sant'Anna Geraldo L
Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Centro de Tecnologia (CT), Laboratory 549-2, Ilha do Fundão, CEP 21945-970, Rio de Janeiro/RJ, Brazil.
Appl Biochem Biotechnol. 2005 Spring;121-124:105-16. doi: 10.1385/abab:121:1-3:0105.
The production of lipase by Penicillium simplicissimum in solid-state fermentation was studied using babassu cake as the basal medium. Tray-type and packed-bed bioreactors were employed. In the former, the influence of temperature; content of the medium, and medium supplementation with olive oil, sugarcane molasses, corn steep liquor, and yeast hydrolysate was studied. For all combinations of supplements, a temperature of 30 degrees C, a moisture content of 70%, and a concentration of carbon source of 6.25% (m/m, dry basis) provided optimum conditions for lipase production. When used as single supplements olive oil and molasses also were able to provide high lipase activities (20 U/g). Using packed-bed bioreactors and molasses-supplemented medium, optimum conditions for enzyme production were air superficial velocities above 55 cm/min and temperatures below 28 degrees C. The lower temperature optimum found for these reactors is probably related to radial heat gradient formation inside the packed bed. Maximum lipase activities obtained in these bioreactors (26.4 U/g) were 30% higher than in tray-type reactors.
以巴巴苏饼为基础培养基，研究了简单青霉在固态发酵中脂肪酶的产生。采用了盘式和填充床生物反应器。在盘式生物反应器中，研究了温度、培养基含量以及培养基添加橄榄油、甘蔗 molasses、玉米浆和酵母水解物的影响。对于所有补充剂组合，30℃的温度、70%的水分含量和6.25%（m/m，干基）的碳源浓度为脂肪酶生产提供了最佳条件。当单独用作补充剂时，橄榄油和 molasses 也能够提供高脂肪酶活性（20 U/g）。使用填充床生物反应器和添加 molasses 的培养基，酶生产的最佳条件是空气表观速度高于55 cm/min 且温度低于28℃。这些反应器中发现的较低温度最佳值可能与填充床内径向热梯度的形成有关。在这些生物反应器中获得的最大脂肪酶活性（26.4 U/g）比盘式反应器高30%。
