Destain Jacqueline, Fickers Patrick, Weekers Frédéric, Moreau Benoît, Thonart Philippe
Centre Wallon de Biologie Industrielle, Unité de Bio-industries, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des Déportés, 2, B-5030 Gembloux, Belgium.
Appl Biochem Biotechnol. 2005 Spring;121-124:269-77. doi: 10.1385/abab:121:1-3:0269.
In this article, we report the development and optimization of an industrial culture medium for the production of extracellular lipase in the yeast Yarrowia lipolytica. Until now olive oil in combination with glucose was used as the carbon source and inducer for the production of lipase. Our results demonstrate that methyloleate, a cheap hydrophobic compound, could efficiently substitute olive oil as the inducer and carbon source for lipase production. A new process of lipase production was developed yielding a twofold increase in the level of production compared with the levels in previous reports.
在本文中，我们报道了用于解脂耶氏酵母中胞外脂肪酶生产的工业培养基的开发与优化。到目前为止，橄榄油与葡萄糖组合被用作脂肪酶生产的碳源和诱导剂。我们的结果表明，廉价的疏水化合物油酸甲酯可以有效地替代橄榄油作为脂肪酶生产的诱导剂和碳源。开发了一种新的脂肪酶生产工艺，与之前报道的水平相比，产量提高了两倍。
