Kiho T, Katsuragawa M, Nagai K, Ukai S, Haga M
Gifu Pharmaceutical University, Japan.
Carbohydr Res. 1992 Feb 7;224:237-43. doi: 10.1016/0008-6215(92)84109-6.
A beta-(1----6)-branched (1----3)-beta-D-glucan(AM-ASN) was isolated from the alkaline extract of the fruiting bodies of Amanita muscaria. AM-ASN had [alpha]D - 11 degrees in 0.5 M sodium hydroxide. Its estimated molecular weight was 95,000 in this alkaline solution and 260,000 in a neutral solution. The branches in the glucan were primarily single, (1----6)-linked D-glucopyranosyl groups, two for every seven residues in the (1----3)-linked main chain. AM-ASN exhibited significant antitumor activity against Sarcoma 180 in mice, and a mixture of AM-ASN with mitomycin C was more effective against the tumor than mitomycin C only.
从毒蝇伞子实体的碱性提取物中分离出一种β-(1→6)分支的(1→3)-β-D-葡聚糖（AM-ASN）。AM-ASN在0.5 M氢氧化钠中的比旋光度[α]D为 -11°。在该碱性溶液中其估计分子量为95,000，在中性溶液中为260,000。葡聚糖中的分支主要是单个的、(1→6)连接的D-吡喃葡萄糖基，在(1→3)连接的主链中每七个残基有两个。AM-ASN对小鼠肉瘤180表现出显著的抗肿瘤活性，并且AM-ASN与丝裂霉素C的混合物对肿瘤的治疗效果比单独使用丝裂霉素C更有效。
