Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-980, Curitiba, PR, Brazil.
Carbohydr Polym. 2013 Oct 15;98(1):761-9. doi: 10.1016/j.carbpol.2013.06.061. Epub 2013 Jul 1.
A fucomannogalactan (FMG-Am) and a (1→3), (1→6)-linked β-D-glucan (βGLC-Am) were isolated from Amanita muscaria fruiting bodies. These compounds' structures were determined using mono- and bi-dimensional NMR spectroscopy, methylation analysis, and controlled Smith degradation. FMG-Am was shown to be a heterogalactan formed by a (1→6)-linked α-D-galactopyranosyl main chain partially substituted at O-2 mainly by α-L-fucopyranose and a minor proportion of β-D-mannopyranose non-reducing end units. βGLC-Am was identified as a (1→3)-linked β-D-glucan partially substituted at O-6 by mono- and a few oligosaccharide side chains, which was confirmed after controlled Smith degradation. Both the homo- and heteropolysaccharide were evaluated for their anti-inflammatory and antinociceptive potential, and they produced potent inhibition of inflammatory pain, specifically, 91±8% (30 mg kg(-1)) and 88±7% (10 mg kg(-1)), respectively.
从鹅膏菌属蕈类的子实体中分离得到了一种岩藻甘露糖聚糖（FMG-Am）和一种（1→3），（1→6）-连接的β-D-葡聚糖（βGLC-Am）。使用单糖和双糖二维 NMR 光谱、甲基化分析和受控 Smith 降解法确定了这些化合物的结构。FMG-Am 被证明是一种杂半乳聚糖，由（1→6）连接的α-D-半乳糖吡喃糖主链组成，该主链在 O-2 位主要被α-L-岩藻糖取代，并且存在少量的非还原端β-D-甘露糖单元。βGLC-Am 被鉴定为（1→3）连接的β-D-葡聚糖，其在 O-6 位被单糖和几个寡糖侧链取代，这在受控 Smith 降解后得到证实。这两种均聚糖都评估了其抗炎和镇痛潜力，它们对炎症性疼痛产生了强大的抑制作用，分别为 91±8%（30 mg kg(-1)) 和 88±7%（10 mg kg(-1))。
