Azmi Asfar Sohail, Bhat Showket Hussain, Hadi S M
Department of Biochemistry, Faculty of Life Sciences, A.M.U. Aligarh 202 002, UP, India.
FEBS Lett. 2005 Jun 6;579(14):3131-5. doi: 10.1016/j.febslet.2005.04.077.
Resveratrol (3,4',5-trihydroxy stilbene), a plant derived polyphenol found in mulberries, grapes and red wine is considered to possess chemopreventive properties against cancer. It is recognized as a naturally occurring antioxidant but also catalyzes oxidative DNA degradation in vitro in the presence of transition metal ions such as copper. Using a cellular system of lymphocytes isolated from human peripheral blood and Comet assay, we have confirmed that resveratrol-Cu(II) system is indeed capable of causing DNA degradation in cells such as lymphocytes. Also, trans-stilbene, which does not have any hydroxyl groups, is inactive in the lymphocyte system. Pre-incubation of lymphocytes with resveratrol indicates that it is capable of either traversing the cell membrane or binding to it. Our results are in partial support of our hypothesis that anticancer properties of various plant derived polyphenols may involve mobilization of endogenous copper and the consequent prooxidant action.
白藜芦醇（3,4',5-三羟基芪）是一种存在于桑葚、葡萄和红酒中的植物源多酚，被认为具有抗癌化学预防特性。它被公认为一种天然抗氧化剂，但在铜等过渡金属离子存在的情况下，在体外也能催化氧化性DNA降解。利用从人外周血分离的淋巴细胞细胞体系和彗星试验，我们证实白藜芦醇 - 铜（II）体系确实能够在淋巴细胞等细胞中引起DNA降解。此外，没有任何羟基的反式芪在淋巴细胞体系中无活性。淋巴细胞与白藜芦醇预孵育表明它能够穿过细胞膜或与之结合。我们的结果部分支持了我们的假设，即各种植物源多酚的抗癌特性可能涉及内源性铜的动员以及随之而来的促氧化作用。
