Bezmialem Vakif University, Istanbul, Turkey.
Advanced Research and Application Center of Traditional and Complementary Medicine, Istanbul, Turkey.
Integr Cancer Ther. 2019 Jan-Dec;18:1534735419876334. doi: 10.1177/1534735419876334.
Many studies have shown that honey with high phenolic contents prevents cancer formation. Furthermore, recent studies have demonstrated that honey can be used for the treatment of cancer as well as cancer prevention. Antineoplastic effects of honey are often associated with their antioxidant phenolic contents. However, very few studies have dealt with the association of phenolic contents of honeys in terms of antiproliferative effects. The aim of this study was, therefore, to elucidate the cytotoxic, genotoxic, apoptotic, and reactive oxygen species (ROS) generating effects of honey samples on the basis of their phenolic and flavonoid contents. Fourteen different honey varieties were collected from various parts of Turkey, and their characteristics regarding total phenols, flavonoids, and antioxidant contents were determined to test their effects on gastric cancer cells (AGS). For convenience, 2 honey varieties were selected, namely, Ida Mountains honeydew honey (QPHH-IM) having the highest phenolic and antioxidant content and Canakkale multifloral honey (MFH-C) with the lowest phenolic and antioxidant content. Levels of 11 different phenolic compounds in QPHH-IM and MFH-C samples were determined by LC-MS/MS. AGS cells were incubated with different concentrations of QPHH-IM and MFH-C for 24 hours, then the cell viability, DNA damage, apoptosis, and generation of ROS were determined. We found that QPHH-IM had more cytotoxic, genotoxic, and apoptotic effects than that of MFH-C. We think that these effects are probably related to pro-oxidant activities due to the high phenolic contents present. Therefore, further research on high-phenolic honey may contribute to the future development of cancer therapeutics.
许多研究表明，酚类含量高的蜂蜜可以预防癌症的形成。此外，最近的研究表明，蜂蜜不仅可以用于癌症的治疗，也可以用于癌症的预防。蜂蜜的抗肿瘤作用通常与其抗氧化的酚类含量有关。然而，很少有研究涉及蜂蜜酚类含量与抗增殖作用之间的关系。因此，本研究旨在根据蜂蜜的酚类和类黄酮含量，阐明蜂蜜样品的细胞毒性、遗传毒性、细胞凋亡和活性氧（ROS）生成作用。从土耳其各地收集了 14 种不同的蜂蜜品种，并测定了其总酚类、类黄酮和抗氧化含量的特征，以测试它们对胃癌细胞（AGS）的影响。为了方便起见，选择了 2 种蜂蜜品种，即酚类和抗氧化含量最高的 Ida 山蜂蜜（QPHH-IM）和酚类和抗氧化含量最低的 Canakkale 百花蜂蜜（MFH-C）。通过 LC-MS/MS 测定了 QPHH-IM 和 MFH-C 样品中 11 种不同酚类化合物的含量。AGS 细胞用不同浓度的 QPHH-IM 和 MFH-C 孵育 24 小时，然后测定细胞活力、DNA 损伤、细胞凋亡和 ROS 的产生。我们发现 QPHH-IM 比 MFH-C 具有更强的细胞毒性、遗传毒性和细胞凋亡作用。我们认为这些作用可能与高酚类含量引起的促氧化剂活性有关。因此，对高酚类蜂蜜的进一步研究可能有助于癌症治疗的未来发展。
