Sheng Yan, Zheng Weihong, Pei Kequan, Ma Keping
Laboratory of Quantitative Vegetation Ecology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Ann Bot. 2005 Aug;96(2):245-52. doi: 10.1093/aob/mci171. Epub 2005 May 26.
China is one of the countries most severely affected by desertification. Haloxylon ammodendron (Amaranthaceae) is an ecologically important component of the desert ecosystem and is one of the main tree species used for restoration, yet we know little about its genetic structure.
Genetic variation within and between nine populations of H. ammodendron from two regions of China was investigated using ISSR (inter-simple sequence repeat) markers.
Eight primers used in this study amplified 219 reproducible bands of which 184 (84 %) were polymorphic. Analysis of molecular variance (AMOVA) revealed high genetic variation within populations (97.63 %) and low genetic differentiation between regions (0.62 %) and among populations (1.75 %).
It is suggested that the present genetic structure could have arisen by high levels of gene flow. The gene flow among populations observed here is probably mainly attributable to pollen movement. The genetic structure also has important implications in ecological restoration practice.
中国是受荒漠化影响最严重的国家之一。梭梭（藜科）是沙漠生态系统中具有重要生态意义的组成部分，也是用于生态恢复的主要树种之一，但我们对其遗传结构知之甚少。
利用ISSR（简单序列重复区间）标记对来自中国两个地区的9个梭梭种群内部和种群之间的遗传变异进行了研究。
本研究使用的8条引物扩增出219条可重复条带，其中184条（84%）具有多态性。分子方差分析（AMOVA）显示，种群内部存在较高的遗传变异（97.63%），地区之间（0.62%）和种群之间（1.75%）的遗传分化较低。
研究表明，目前的遗传结构可能是由高水平的基因流形成的。这里观察到的种群间基因流可能主要归因于花粉传播。这种遗传结构在生态恢复实践中也具有重要意义。
