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[recB和recA突变对大肠杆菌各种修饰-限制质粒系统中噬菌体限制作用的影响]

[Effect of recB- and recA-mutations on phage restriction in various modification-restriction plasmid systems of E. coli].

作者信息

Torosian M V, Shishkova O V, Rabinkova E V, Fradkin G E

出版信息

Mol Gen Mikrobiol Virusol. 1988 May(5):36-41.

PMID:2842671
Abstract

The effect of recB and recA mutations on lambda vir and P1 vir restriction by different restriction-modification plasmid systems of E. coli was studied. It was shown that effect of R1 plasmid coded restriction-modification in E. coli K12 and E. coli B strains and pJA4620 plasmid coded restriction in E. coli K12 is observed only in RecB+ strain. Phenomenon of restriction-modification determined by R124, R245 plasmids does not depend of recB mutation. Effect of recA mutation has not been found in cultures harbouring R1, R245, R124 pJA4620 plasmids.

摘要

研究了recB和recA突变对大肠杆菌不同限制修饰质粒系统对λ噬菌体vir和P1噬菌体vir的限制作用。结果表明,R1质粒编码的限制修饰在大肠杆菌K12和大肠杆菌B菌株中的作用以及pJA4620质粒编码的限制在大肠杆菌K12中的作用仅在RecB+菌株中观察到。由R124、R245质粒决定的限制修饰现象不依赖于recB突变。在含有R1、R245、R124和pJA4620质粒的培养物中未发现recA突变的影响。

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Mol Gen Mikrobiol Virusol. 1988 May(5):36-41.
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