通过等电聚焦和免疫印迹法在12个群体中对血浆锌α2-糖蛋白变体的5个新等位基因进行表型分析。

Five new alleles of plasma Zn-alpha 2-glycoprotein variants phenotyped by isoelectric focusing and immunoblotting in twelve populations.

作者信息

Nakayashiki N, Umetsu K, Yuasa I, Suenaga K, Omoto K, Ishida T, Misawa S, Katsura S

机构信息

Department of Legal Medicine, Iwate Medical University School of Medicine, Morioka, Japan.

出版信息

Electrophoresis. 1992 Mar;13(3):154-7. doi: 10.1002/elps.1150130131.

DOI:10.1002/elps.1150130131

PMID:1592045

Abstract

Human Zn-alpha 2-glycoprotein (ZAG) in plasma samples from twelve populations was tested by immunoblotting after polyacrylamide gel isoelectric focusing. Eleven ZAG phenotypes produced by one common and nine rare alleles, including five new ones (ZAG6-ZAG10), were detected. Additionally, an application of separator IEF with N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) was found to be useful for discriminating the rare ZAG 7 band.

摘要

通过聚丙烯酰胺凝胶等电聚焦后免疫印迹法检测了来自十二个群体的血浆样本中的人锌α2糖蛋白（ZAG）。检测到由一个常见等位基因和九个稀有等位基因产生的11种ZAG表型，包括五个新的等位基因（ZAG6-ZAG10）。此外，发现使用含N-2-羟乙基哌嗪-N'-2-乙磺酸（HEPES）的分离等电聚焦法有助于区分罕见的ZAG 7条带。