通过等电聚焦和免疫印迹法对血迹中的α-2-HS糖蛋白进行表型分析。

Phenotyping of alpha-2-HS glycoprotein in bloodstains by isoelectric focusing and immunoblotting.

作者信息

Thomas A S

出版信息

J Forensic Sci Soc. 1989 Sep-Oct;29(5):325-30. doi: 10.1016/s0015-7368(89)73272-2.

Abstract

A sensitive immunoblotting procedure has been applied to the detection of alpha-2-HS-glycoprotein (A2HS) phenotypes from control and casework bloodstains. A2HS phenotypes were separated by thin layer polyacrylamide gel isoelectric focusing (PAGIEF) in gels containing Pharmalyte pH 4.2-4.9. After transfer to nitrocellulose by a rapid capillary blot, the A2HS phenotypes were developed using a double antibody enzyme-immunoassay. The evaluation of A2HS phenotyping of casework material was undertaken in parallel with phosphoglucomutase (PGM) phenotyping by PAGIEF. A total of 598 water extracts from casework bloodstains have been tested. Positive results were obtained in 84% and 75% of samples for PGM and A2HS respectively. The A2HS gene frequencies A2HS1 = 0.6420, A2HS2 = 0.3530, and A2HS*3 = 0.0050 were determined from a survey of 1000 people in Brisbane.

摘要

一种灵敏的免疫印迹法已应用于检测对照和实际案件血迹中的α-2-HS-糖蛋白（A2HS）表型。A2HS表型通过在含有pH 4.2 - 4.9 Pharmalyte的凝胶中进行薄层聚丙烯酰胺凝胶等电聚焦（PAGIEF）来分离。通过快速毛细管印迹转移至硝酸纤维素膜后，使用双抗体酶免疫测定法显影A2HS表型。实际案件材料的A2HS表型分析与通过PAGIEF进行的磷酸葡萄糖变位酶（PGM）表型分析同时进行。总共对598份实际案件血迹的水提取物进行了检测。PGM和A2HS样本的阳性结果分别为84%和75%。通过对布里斯班1000人的调查确定A2HS基因频率为A2HS1 = 0.6420，A2HS2 = 0.3530，以及A2HS*3 = 0.0050。