乳酸克鲁维酵母低氧基因KlHEM13的功能表征
Functional characterization of KlHEM13, a hypoxic gene of Kluyveromyces lactis.
作者信息
Blanco Moisés, Becerra Manuel, González-Siso M Isabel, Cerdán M Esperanza
机构信息
Departmento de Biología Celular y Molecular, Universidad de A Coruña, F. Ciencias, Spain.
出版信息
Can J Microbiol. 2005 Mar;51(3):241-9. doi: 10.1139/w04-133.
The KlHEM13 gene of Kluyveromyces lactis encoding the coproporphyrinogen oxidase (EC 1.3.3.3), an oxygen-requiring enzyme that catalyzes the sixth step of heme biosynthesis, was cloned and functionally characterized. The coding and upstream regions of KlHEM13 were analyzed and the putative cis regulatory elements were discussed in relation to the mechanisms of regulation of this hypoxic gene in K. lactis.
乳酸克鲁维酵母（Kluyveromyces lactis）的KlHEM13基因编码粪卟啉原氧化酶（EC 1.3.3.3），这是一种需氧酶，催化血红素生物合成的第六步，该基因已被克隆并进行了功能表征。分析了KlHEM13的编码区和上游区域，并结合乳酸克鲁维酵母中该缺氧基因的调控机制讨论了推定的顺式调控元件。
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