Wilson Paul E, Alker Alisa P, Meshnick Steven R
University of North Carolina School of Medicine, Department of Microbiology and Immunology, CB 7290, Chapel Hill, NC 27599, USA.
Trends Parasitol. 2005 Jun;21(6):278-83. doi: 10.1016/j.pt.2005.04.007.
Drug-resistant Plasmodium falciparum is a challenge to malaria control programs. Policy makers currently depend on in vivo (and, sometimes, in vitro) resistance testing to set treatment guidelines. Molecular markers such as mutations in dhfr, dhps, pfcrt and pfmdr1 represent potential surveillance tools. In this article, we describe newer high-throughput methods for detecting these molecular markers. One method, 5' nuclease real-time polymerase chain reaction, is discussed in detail.
耐药性恶性疟原虫是疟疾控制项目面临的一项挑战。政策制定者目前依赖体内（有时也包括体外）耐药性检测来制定治疗指南。二氢叶酸还原酶（dhfr）、二氢蝶酸合成酶（dhps）、氯喹抗性转运蛋白（pfcrt）和疟原虫多药耐药蛋白1（pfmdr1）中的突变等分子标记代表了潜在的监测工具。在本文中，我们描述了用于检测这些分子标记的更新的高通量方法。其中一种方法，即5'核酸酶实时聚合酶链反应，将进行详细讨论。
