Zhou Fengfeng, Xue Yu, Lu Hualei, Chen Guoliang, Yao Xuebiao
National High-Performance Computing Center at Hefei, University of Science and Technology of China, Hefei 230027, China.
FEBS Lett. 2005 Jun 20;579(16):3369-75. doi: 10.1016/j.febslet.2005.04.076.
Protein sumoylation is an important reversible post-translational modification of proteins in the nucleus, and it orchestrates a variety of the cellular processes. Genome-wide analysis of functional abundance and distribution of Small Ubiquitin-related MOdifier (SUMO) substrates may shed a light on how sumoylation is involved in nuclear biological processes and functions. Two interesting questions about sumoylation have emerged: (1) how many SUMO substrates exist in mammalian proteomes, such as human and mouse, (2) and what are their functions and how are they involved in a variety of biological processes? To address these two questions,we present an in silico genome-scale analysis for SUMO substrates in human. Based on the pattern recognition and phylogenetic conservation, we retrieved a list of 2683 potential SUMO substrates conserved in both human and mouse. Then, by functional enrichment analysis, we surveyed the over-represented GO terms and functional domains of them against the whole human proteome. Besides the consistence between our analyses and in vivo or in vitro work, the in silico predicted candidates also point to several potential roles of sumoylation, e.g., perception of sound. These potential SUMO substrates in human are of great value for further in vivo or in vitro experimental analysis.
蛋白质SUMO化是细胞核中蛋白质重要的可逆性翻译后修饰，它调控着多种细胞过程。对小泛素相关修饰物（SUMO）底物的功能丰度和分布进行全基因组分析，可能会揭示SUMO化如何参与核生物学过程和功能。关于SUMO化出现了两个有趣的问题：（1）在哺乳动物蛋白质组中，如人类和小鼠，存在多少种SUMO底物，（2）它们的功能是什么，以及它们如何参与各种生物学过程？为了解决这两个问题，我们对人类中的SUMO底物进行了计算机模拟的全基因组规模分析。基于模式识别和系统发育保守性，我们获得了一份在人类和小鼠中均保守的2683种潜在SUMO底物的列表。然后，通过功能富集分析，我们针对整个人类蛋白质组调查了它们中过度富集的基因本体（GO）术语和功能结构域。除了我们的分析与体内或体外研究结果一致外，计算机模拟预测的候选物还指出了SUMO化的几个潜在作用，例如声音感知。人类中的这些潜在SUMO底物对于进一步的体内或体外实验分析具有重要价值。
