Kubo Takuya, Sano Tomoharu, Hosoya Ken, Tanaka Nobuo, Kaya Kunimitsu
Graduate School of Environmental Studies, Tohoku University, Aramaki-aza aoba 6-6-20, Aoba-ku, Sendai 980-8579, Japan.
Toxicon. 2005 Jul;46(1):104-7. doi: 10.1016/j.toxicon.2005.03.020.
A new simply and effective fractionation method for cylindrospermopsin (CYN) analyses was developed. The extract from cells of Cylindrospermopsis raciborskii was resuspended with 0.1 M carbonate buffer at pH 10.5, and pass through the double-cartridges column which was consisted of a styrene polymer cartridge and an anion exchange cartridge. CYN and deoxy-CYN were adsorbed with the anion exchange cartridge. After separation of the anion exchange cartridge, adsorbed compounds were eluted from the cartridge with 50% methanol containing 1% formic acid solution. CYN and deoxy-CYN were selectively condensed in the eluted solution. When CYN was analyzed by LC-photodiode array or LC/MS, only two peaks of CYN and deoxy-CYN were detected quantitatively. The results suggest that the fractionation method is a useful method for CYN analyses and must be utilized for CYN purification.
