Bureau of Chemical Safety, Health Products and Food Branch, Health Canada, Ottawa, Ontario, Canada.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011 Jun;28(6):786-90. doi: 10.1080/19440049.2010.501824.
For the analysis of blue-green algal food supplements for cylindrospermopsin (CYN), a C18 solid-phase extraction column and a polygraphitized carbon solid-phase extraction column in series was an effective procedure for the clean-up of extracts. Determination of CYN was by liquid chromatography with ultraviolet light detection. At extract spiking levels of CYN equivalent to 25-500 µg g(-1), blue-green algal supplement recoveries were in the range 70-90%. CYN was not detected in ten samples of food supplements and one chocolate product, all containing blue-green algae. The limit of detection for the method was 16 µg g(-1), and the limit of quantification was 52 µg g(-1).
对于蓝绿藻食品补充剂中筒状海丝菌素（CYN）的分析，C18 固相萃取柱和石墨化碳固相萃取柱串联是一种有效的提取净化程序。CYN 的测定采用液相色谱法和紫外光检测。在 CYN 相当于 25-500 µg g(-1)的提取物加标水平下，蓝绿藻补充剂的回收率在 70-90%范围内。十种食品补充剂和一种巧克力产品均未检出 CYN，这些产品均含有蓝绿藻。该方法的检测限为 16 µg g(-1)，定量限为 52 µg g(-1)。
