Good Michael F
The Queensland Institute of Medical Research, PO Royal Brisbane Hospital, Brisbane 4029, Australia.
Trends Immunol. 2005 Jun;26(6):295-7. doi: 10.1016/j.it.2005.04.005.
A genetically modified malaria sporozoite might breathe new life into the traditional approach to vaccine development, that of using whole organisms. Mueller and colleagues recently knocked out a gene, UIS3, from the rodent parasite, Plasmodium berghei, and demonstrated that the sporozoite forms could not develop beyond the stage of the life cycle in the liver (thus not giving rise to clinical disease, which is associated with blood infection) but could induce protection against subsequent challenge with genetically intact sporozoites. UIS3(-) sporozoites or irradiated sporozoites might find success where subunit approaches are struggling.
一种经过基因改造的疟原虫子孢子可能会给传统的疫苗开发方法——即使用全生物体的方法——注入新的活力。穆勒及其同事最近从啮齿动物寄生虫伯氏疟原虫中敲除了一个名为UIS3的基因，并证明子孢子形式在肝脏中的生命周期阶段之后无法发育（因此不会引发与血液感染相关的临床疾病），但可以诱导对随后用基因完整的子孢子进行攻击的保护作用。在亚单位方法面临困境的情况下，UIS3（-）子孢子或经辐射的子孢子可能会取得成功。
