Pache Mona, Zieger Barbara, Bläser Susanne, Meyer Peter
University Eye Clinic and Department of Pediatrics and Adolescent Medicine, University of Freiburg, Freiburg, Germany.
J Histochem Cytochem. 2005 Sep;53(9):1139-47. doi: 10.1369/jhc.4A6588.2005. Epub 2005 May 27.
We aimed to examine the distribution of SEPT4, SEPT5, and SEPT8 in the human eye. For each septin, five to six normal human eyes were examined by immunohistochemical staining of paraffin sections using polyclonal antibodies against SEPT4, SEPT5, and SEPT8 and an avidin biotin complex immunodetection system. SEPT4 immunoreactivity (IR) was detected primarily in the epithelium of cornea, lens, and nonpigmented ciliary epithelium; in the endothelium of cornea and vessels of iris and retina; and in the retinal nerve fiber layer, the outer plexiform layer, the outer segments of the photoreceptor cells, the inner limiting membrane of the optic nerve head, and optic nerve axons. SEPT5-IR was present in corneal endothelial cells, iris tissue, nonpigmented ciliary epithelium, and epithelial cells of the lens. SEPT8-IR almost paralleled that of SEPT4, except for a lower SEPT8-IR of the outer photoreceptor segments and a positive staining of the meningothelial cell nests in the subarachnoidal space of the bulbar segment of the orbital optic nerve. In conclusion, SEPT4, SEPT5, and SEPT8 are expressed in various ocular tissues, each revealing a distinct expression pattern. Both physiological and potential pathophysiological role of septins in the human eye deserve further investigation.
我们旨在研究SEPT4、SEPT5和SEPT8在人眼中的分布情况。对于每种septin，使用针对SEPT4、SEPT5和SEPT8的多克隆抗体以及抗生物素蛋白生物素复合物免疫检测系统，通过石蜡切片的免疫组织化学染色对五到六只正常人眼进行了检查。SEPT4免疫反应性（IR）主要在角膜、晶状体和无色素睫状上皮的上皮细胞中检测到；在角膜内皮以及虹膜和视网膜血管中；以及在视网膜神经纤维层、外网状层、光感受器细胞的外段、视神经乳头的内界膜和视神经轴突中。SEPT5-IR存在于角膜内皮细胞、虹膜组织、无色素睫状上皮和晶状体上皮细胞中。SEPT8-IR几乎与SEPT4的平行，但光感受器外段的SEPT8-IR较低，并且在眶部视神经球部段蛛网膜下腔的脑膜内皮细胞巢有阳性染色。总之，SEPT4、SEPT5和SEPT8在各种眼组织中表达，各自呈现出独特的表达模式。septin在人眼中的生理和潜在病理生理作用值得进一步研究。
