Helmprobst Frederik, Lillesaar Christina, Stigloher Christian
Biocenter, Division of Electron Microscopy, University of Würzburg Würzburg, Germany.
Biocenter, Department of Physiological Chemistry, University of Würzburg Würzburg, Germany.
Front Neuroanat. 2017 Feb 17;11:6. doi: 10.3389/fnana.2017.00006. eCollection 2017.
Septins are a highly conserved family of small GTPases that form cytoskeletal filaments. Their cellular functions, especially in the nervous system, still remain largely enigmatic, but there are accumulating lines of evidence that septins play important roles in neuronal physiology and pathology. In order to further dissect septin function in the nervous system a detailed temporal resolved analysis in the genetically well tractable model vertebrate zebrafish () is crucially necessary. To close this knowledge gap we here provide a reference dataset describing the expression of selected septins (, and ) in the zebrafish central nervous system. Strikingly, proliferation zones are devoid of expression of all three septins investigated, suggesting that they have a role in post-mitotic neural cells. Our finding that three septins are mainly expressed in non-proliferative regions was further confirmed by double-stainings with a proliferative marker. Our RNA hybridization (ISH) study, detecting , and mRNAs, shows that all three septins are expressed in largely overlapping regions of the developing brain. However, the expression of is much more confined compared to and . In contrast, the expression of all the three analyzed septins is largely similar in the adult brain.
Septins是一类高度保守的小GTP酶家族，可形成细胞骨架细丝。它们的细胞功能，尤其是在神经系统中的功能，在很大程度上仍然是个谜，但越来越多的证据表明Septins在神经元生理和病理过程中发挥着重要作用。为了进一步剖析Septins在神经系统中的功能，在遗传上易于处理的模式脊椎动物斑马鱼中进行详细的时间分辨分析至关重要。为了填补这一知识空白，我们在此提供了一个参考数据集，描述了斑马鱼中枢神经系统中选定的Septins（、和）的表达情况。令人惊讶的是，增殖区没有所研究的所有三种Septins的表达，这表明它们在有丝分裂后的神经细胞中发挥作用。我们用增殖标记进行的双重染色进一步证实了我们的发现，即三种Septins主要在非增殖区域表达。我们检测、和mRNA的RNA杂交（ISH）研究表明，所有三种Septins在发育中的大脑的大部分重叠区域表达。然而，与和相比，的表达范围要窄得多。相比之下，在成体大脑中，所有三种分析的Septins的表达在很大程度上是相似的。
